A rapid and sensitive liquid chromatography–tandem mass spectrometry (LC-MS) method was developed for the determination of enalapril and enalaprilat in human plasma. Detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (ESI) interface in positive ion mode which was operated under the multiple-reaction monitoring mode. Sample pretreatment was involved...

a rapid and sensitive liquid chromatography–tandem mass spectrometry (lc-ms) method for the estimation of enalapril and enalaprilat in human plasma. detection of analytes was achieved by tandem mass spectrometry with electrospray ionization (esi) interface in positive ion mode was operated under the multiple-reaction monitoring mode. sample pretreatment involved in a one-step protein precipitat...

tions. J Chromatogr 1992;577:205–14. 11. Matsumoto I, Kuhara T. Inborn errors of amino acid and organic acid metabolism. In: Desiderio DM, ed. Mass spectrometry: clinical and biomedical applications, Vol. I. New York: Plenum Press, 1992:259–98. 12. Chace DH. Mass spectrometry in the clinical laboratory. Chem Rev 2001; 101:445–77. 13. Millington D, Chace D. Carnitine and acylcarnitines in metabo...

Accurate assignment of peptide sequences to observed fragmentation spectra is hindered by the large number of hypotheses that must be considered for each observed spectrum. A high score assigned to a particular peptide-spectrum match (PSM) may not end up being statistically significant after multiple testing correction. Researchers can mitigate this problem by controlling the hypothesis space i...

Several approaches to ion activation in tandem mass spectrometry have been developed in recent years for use in ion trapping instruments that allow for conditions to be reached wherein rates of ion activation and deactivation are comparable. These approaches are deÐned as slow heating methods and include continuous-wave laser infrared multiphoton dissociation, dissociation driven by blackbody r...

Metabolomics is a rapidly expanding field with applications in areas such as medicine, agriculture, or food safety. Tandem mass spectrometry (MS) is one of the main technologies that drives the field forward. Optionally coupled to a chromatographic element, MS can capture detailed snapshots of an organism’s metabolome. The resulting data sets are complex and difficult to analyse due to the mult...

A new tandem time-of-flight (TOF-TOF) instrument has been developed by modifying a standard matrix-assisted laser desorption ionization (MALDI)-TOF instrument to make high-performance, high-energy collision-induced dissociation (CID) MALDI tandem mass spectrometry (MS) a practical reality. To optimize fragment spectra quality, the selected precursor ion is decelerated before entering a floating...

In order to assist with the development of more selective and sensitive methods for thyroid hormone analysis the [M-H]- anions of the iodothyronines T4, T3, rT3, (3,5)-T2 and the non-iodinated thyronine (T0) have been generated by negative ion electrospray mass spectrometry. Tandem mass spectra of these ions were recorded on a triple-quadrupole mass spectrometer and show a strong analogy with t...

PAGE gel, individual proteins were either visualized by Coomassie Brilliant Blue stain excised and subjected to an in-gel tryptic digest (digests were performed as described (Shevchenko et al, 1996), or the eluted CenH3 particles were precipitated with 10% trichloroacetic acid, washed once with acetone, dried, re-dissolved in de-ionized water, and digested in solution with Arg-C protease. For C...

BACKGROUND New high-performance liquid chromatography/tandem mass spectrometry (LC-MS/MS) methods are among the most successful approaches to improve specificity problems inherent in many immunoassays. CONTENT We emphasize problems with immunoassays for the measurement of steroids and review the emerging role of LC-MS/MS in the measurement of clinically relevant steroids. The latest generatio...