Li, X. 2018. Development of robust cyanobacterial strains for biotechnological applications. Stress tolerance and cell-specific expression in heterocyst-forming cyanobacteria. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 1641. 80 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0259-1. Synthetic biology tools can be used to expl...

background: the organochlorine ddt has estrogenic activity but the mechanism underlying the estrogenic activity of this pesticide remains unclear. in the present investigation here, we studied the transcriptional effects of a synthetic organochlorine pesticide o,p’-ddt [1.1.1.-trichloro-2-(o-chlorophenyl)-2-p-chloriphenyl ethane] and its metabolite p,p'-dde (2-2-bis(4/chlorophenyl)-1-1-dic...

The Ultrabithorax (Ubx) gene of Drosophila melanogaster encodes a family of UBX proteins that are thought to specify the developmental fates of segments in the posterior thorax and anterior abdomen by controlling the expression of a set of target genes. UBX proteins bind DNA in vitro, and they activate or repress different natural and synthetic target promoters in cultured cells. Here it is sho...

The application of synthetic biology requires characterized tools to precisely control gene expression. This toolbox of genetic parts previously did not exist for the industrially promising cyanobacterium, Synechococcus sp. strain PCC 7002. To address this gap, two orthogonal constitutive promoter libraries, one based on a cyanobacterial promoter and the other ported from Escherichia coli, were...

Synthetic biology has developed numerous parts for building synthetic gene circuits. However, few parts have been described for prokaryotes to integrate two signals at a promoter in an AND fashion, i.e. the promoter is only activated in the presence of both signals. Here we present a new part for this function: a split intein T7 RNA polymerase. We divide T7 RNA polymerase into two expression do...

Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo and the results are discussed with respect to the consensus sequence and other promoter mutations located in this region.

Starting from a synthetic E. coli promoter with the consensus sequences at -35 and -10 regions, a sequence CAT frequently occurred in the RNA start points of natural promoters was introduced in the downstream of the consensus sequences, and the sequences around the RNA start points as well as the relative positions of CAT from the consensus sequences were altered. Analysis of the RNA start poin...