We identified a new gene that is interrupted by T-DNA in an Arabidopsis embryo mutant called raspberry3. raspberry3 has "raspberry-like" cellular protuberances with an enlarged suspensor characteristic of other raspberry embryo mutants, and is arrested morphologically at the globular stage of embryo development. The predicted RASPBERRY3 protein has domains found in proteins present in prokaryot...

The immature seeds of Dendrobium nobile acquire resistance to freezing and long-term storage in a cryobank at the age 6 months after pollination. Seed maturation Lindl establish optimal stage for liquid nitrogen was studied. Immature can germinate vitro starting from 3 pollination (MAP) but only develop up embryo swelling or protocorm without rhizoids. maximum staining embryos with vital dyes F...

BACKGROUND The division of plant zygotes is typically asymmetric, generating daughter cells with different developmental fates. In Arabidopsis, the apical daughter cell produces the proembryo, whereas the basal daughter cell forms the mostly extraembryonic suspensor. Establishment of apical and basal fates is known to depend on the YODA (YDA) mitogen-associated protein (MAP) kinase cascade and ...

Formation of chlamydospores by Candida albicans was an established medical diagnostic test to confirm candidiasis before the molecular era. However, the functional role and pathological relevance of this in vitro morphological transition to pathogenesis in vivo remain unclear. We compared the physical properties of in vitro-induced chlamydospores with those of large C. albicans cells purified b...

The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte embryo), proliferating embryonal-suspensor mass (ESM), non-embryogenic callus Pinus sibirica Larix sibirica. Furthermore, the localization hormones cells was studied with immunohistochemical analysis. It shown that formation embryogenic cultures both species is ass...

In the animal life cycle, the earliest manifestations of programmed cell death (PCD) can already be seen during embryogenesis. The aim of this work was to determine if PCD is also involved in the elimination of certain cells during plant embryogenesis. We used a model system of Norway spruce somatic embryogenesis, which represents a multistep developmental pathway with two broad phases. The fir...