BACKGROUND Lipases including the lipase from Burkholderia cepacia are in a main focus in biotechnology research since many years because of their manifold possibilities for application in industrial processes. The application of Burkholderia cepacia lipase for these processes appears complicated because of the need for support by a chaperone, the lipase specific foldase. Purification and recons...

The conditions, including mass ratio of PEG4000 to lipase, pH, and mass ratio of diatomites to lipase, for immobilization of Candida antarctica lipase with PEG non-covalent modification were optimized by means of the response surface methodology (RSM). The immobilized lipase specific activity in the reaction of transesterification was selected as the response value. A mathematical model was dev...

recent progress in nanotechnology and electrochemical methods can be applied to fine control of the size, crystal structure, and surface properties of iron oxide nanoparticles. here we appliedcathodic electrochemical deposition (ced) as an efficient and effective tactic for synthesisand double coating of surface of superparamagnetic iron oxide nanoparticles (spions). in first step, bare fe3o4 n...

Conjugated Linoleic Acid (CLA), Glycerol (G) and sunflower oil blends with varying concentration were subjected to enzymatic esterification using a 1, 3- specific immobilized lipase. CLA was used as acyl due to its purported health benefits. The transesterified lipids were evaluated for free fatty acids (FFA) and composition of fatty acids by gas chromatography. Lipozyme RM IM is preferred for ...

Recent progress in nanotechnology and electrochemical methods can be applied to fine control of the size, crystal structure, and surface properties of iron oxide nanoparticles. Here we appliedcathodic electrochemical deposition (CED) as an efficient and effective tactic for synthesisand double coating of surface of superparamagnetic iron oxide nanoparticles (SPIONs). In first step, bare Fe3O4 n...

background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...

Addition of lard or sodium oleate to the medium used for lipase production by Pseudomonas fragi resulted in a decreased accumulation of lipase in the culture supernatant fluid without affecting cell growth. The production and activity of lipase was inhibited by lard, sodium oleate, and the salts of other unsaturated fatty acids. Some divalent cations, Tweens, lecithin, and bovine serum prevente...

Human milk fat globules were used to explore how dietary triglycerides are hydrolyzed by pancreatic lipase. These triglycerides were hydrolyzed very slowly by lipase alone as if the surface layer of proteins and phospholipids impeded the action of the enzyme. The inhibition of lipase activity could be overcome by addition either of colipase or of pancreatic phospholipase A2. Colipase enhanced t...

VSG lipase of Trypanosoma brucei specifically cleaves the glycosyl-phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein (VSG), releasing this protein from the plasma membrane. It also cleaves similar membrane anchors on some mammalian proteins. VSG lipase may play a role in processes such as parasite differentiation or antigenic variation. We describe here the cl...