The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method for gene expression analysis requires one or several reference gene(s) acting as normalization factor(s). In order to facilitate gene expression studies in sugarcane (Saccharum officinarum), a non-model plant with limited genome information, the stability of 13 candidate reference genes was evaluated. The ge...

Centromeres in eukaryotes are composed of tandem DNAs and retrotransposons. However, centromeric repeats exhibit considerable diversity, even among closely related species, their origin evolution largely unknown. We conducted a genome-wide characterization the sequences sugarcane (Saccharum officinarum). Four repeat sequences, So1, So103, So137 So119, were isolated. So1 has monomeric length 137...

Sugarcane (Saccharum officinarum L.) is an important commercial crop of India. and sugar beet are used for large scale production in the world. The present study was conducted purposively selected Karnal district Haryana state. There 8 blocks Haryana, out which Indri block were on basis maximum area under cultivation sugarcane. From list so prepared, 12 respondents from 10 villages constitutes ...

Cultivated sugarcane genotypes (Saccharum spp.) are derived from complex interspecific hybridisations between the species S. spontaneum and S. officinarum. To analyse this complex genome, we have investigated the potential of sugarcane microsatellite sequences as genetic markers in terms of their abundance, variability and ability to detect polymorphisms. From an enriched sugarcane genornic DNA...

Sugarcane (Saccharum officinarum L.) juice is widely consumed by people of the tropics and subtropics. It has been used to cure jaundice and liver-related disorders in Indian systems of medicine. Its possible mechanism of action was examined in terms of antioxidant availability. The assays involved different levels of antioxidant action such as oxygen radical absorbance capacity (ORAC), radical...

Comparative genetic maps of Papuan Saccharum officinarum L. (2n = 80) and S. robustum (2n = 80) were constructed by using single-dose DNA markers (SDMs). SDM-framework maps of S. officinarum and S. robustum were compared with genetic maps of sorghum and maize by way of anchor restriction fragment length polymorphism probes. The resulting comparisons showed striking colinearity between the sorgh...

Sugarcane (Saccharum officinarum L.) is a multipurpose and commercial crop of Pakistan. Sugar for food remains the most important product of sugarcane, by-products of the crop, many of them from fiber, are a significant part of its economic production. Sucrose serves as the major form of carbohydrate storage in sugarcane. Metabolism of sucrose, a mobile source of energy and carbon, is an absolu...