−− The present work is focused on evaluating the anaerobic digestion of complex substrates (proteins and carbohydrates) in anaerobic fluidized bed reactors (AFBRs). In a previous paper, an AFBR model was adjusted using only the experimental data obtained during the acclimatization stage (glucose and acetate-based feeding) of two mesophilic bioreactors. Here, the sensitivity of that model to rep...

A carboxylic-ester hydrolase was isolated from the leukocytes of a patient with myelomonocytic leukemia. Its relative molecular mass as estimated by sucrose density-gradient sedimentation is about 70 000. The purified enzyme is specific for acetyl esters of aromatic alcohols. It is inhibited by fluoride, but insensitive to eserine or p-chloromercuriphenylsulfonate. Hydrolysis of 1-naphthyl acet...

A new isolation procedure has resulted in an improved yield of stable 68S chloroplast ribosomes from Euglena gracilis var. bacillaris. Chloroplasts are isolated by suspending the cells in buffer I (sorbitol, 250 mm; sucrose, 250 mm; Ficoll, 2.5% [w/v]; magnesium acetate, 1 mm; bovine serum albumin, 0.01% [w/v]; mercaptoethanol, 14 mm; N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid, pH 7.6...

The Food and Drug Administration (FDA) is amending the food additive regulations to provide for the safe use of sucrose acetate isobutyrate (SAIB) as a stabilizer of emulsions of flavoring oils used in nonalcoholic beverages. This action is in response to a petition filed by Eastman Chemical Co. DATES: Effective (inserl date of publication in the Federal Register); written objections and reques...

( Asymmetric cellulose acetate membranes have been prepared and characterized in the laboratory with a view to use them in membrane bio-reactors. Enzymatic hydrolysis of sucrose has been investigated in a dead-end stirred cell type membrane bio-reactor using these membranes. Effect of different operating variables such as substrate concentration, enzyme loading, flow rate and working volume hav...

The activity of the overall pyruvate dehydrogenase complex was found to be similar in extracts of Aspergillus nidulans after growth on either sucrose or acetate. Eight mutants lacking the activity of this complex were found among some 200 glycolytic mutants selected for their inability to grow on sucrose. The absence of pyruvate dehydrogenase complex activity was also confirmed for a mutant, g6...

Endophytic fungi are a source of antioxidant compounds in nature. The low yield extracts and active produced is one the limiting factors for using endophytic as natural compounds. Modification growth media an alternative solution to overcome this problem. This study aimed determine effect conditions, belonging carbon nitrogen sources different initial pH fermentation, on through DPPH radical sc...

Figlewicz DP, Bennett JL, Aliakbari S, Zavosh A, Sipols AJ. Insulin acts at different CNS sites to decrease acute sucrose intake and sucrose self-administration in rats. Am J Physiol Regul Integr Comp Physiol 295: R388–R394, 2008. First published June 4, 2008; doi:10.1152/ajpregu.90334.2008.—Findings from our laboratory and others have demonstrated that the hormone insulin has chronic effects w...