This study investigated the effect of Bacillus subtilis (B. subtilis) natto on meat quality and skatole in TOPIGS pigs. Sixty TOPIGS pigs were randomly assigned to 3 groups (including 5 pens per group, with 4 pigs in each pen) and fed with basic diet (control group), basic diet plus 0.1% B. subtilis natto (B group), and basic diet plus 0.1% B. subtilis natto plus 0.1% B. coagulans (BB group), r...

Preparations of Bacillus subtilis RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) from vegetatively growing cells contain small amounts of an activity (B. subtilis RNA polymerase holoenzyme II) that shows a unique promoter specificity with T7 bacteriophage DNA as compared with the normal B. subtilis holoenzyme (holoenzyme I) and lacks the normal sigma subunit [Jae...

Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identified the RLP of B. subtilis as the 2,3-diketo-5-methylthiopentyl-1-phosphate en...

objective: organophosphorus (ops) compounds are widely used in many pesticides, insecticides and chemical nerve agents. these compounds are hazardous for humans and the environment. organophosphate hydrolase (oph) is a homodimeric protein initially isolated from pseudomonas diminuta mg and flavobacterium species. this enzyme is able to degrade a broad spectrum of toxic ops compounds. using immo...

background: degradation of phytic acid to inorganic phosphate in domestic animals’ diets requires thermostable phytase. although basillus subtilis phytase shows a potential to be degraded phytate complex in high temperature, the enzyme activities and yields need to be increased to make them possible for industrial application. methods: the phytase gene from bacillus subtilis dr8886 was isolated...

abstract background and aims: influenza a(h5n1) viruses  circulating in animals might evolve and acquire the ability to spread from  human to human and thus start a pandemic. hemagglutinin (ha) has been shown to play a major role in binding of influenza virus to its target cell and the main neutralizing antibody responses elicit against this region. recent studies have shown that glycosylation ...

background and aims: influenza a(h5n1) viruses  circulating in animals might evolve and acquire the ability to spread from  human to human and thus start a pandemic. hemagglutinin (ha) has been shown to play a major role in binding of influenza virus to its target cell and the main neutralizing antibody responses elicit against this region. recent studies have shown that glycosylation of ha is ...

lipases catalyze hydrolysis and synthesis of triacylglycerols. these enzymes,especially the microbial ones, have wide commercial and industrial usage. bacillus subtilis lipase has interesting properties such as small size and tolerance to basic ph; therefore, developing techniques for its recognition and isolation is the focus of research in many laboratories. in the present study, two factors ...

A partial amino-acid sequence of Bacillus subtilis 168 flagellin is presented. The region of unassigned sequence in this 304-residue polypeptide chain spans residues 158-173. Comparison of the 27-residue aminoterminal CNBr peptide of B. subtilis 168 flagellin with that derived from the flagellin of the serologically unrelated strain of B. subtilis, W23, shows only three conservative substitutio...

The comQXPA locus of Bacillus subtilis encodes a quorum sensing (QS) system typical of Gram positive bacteria. It encodes four proteins, the ComQ isoprenyl transferase, the ComX pre-peptide signal, the ComP histidine kinase, and the ComA response regulator. These are encoded by four adjacent genes all situated on the same chromosome strand. Here we present results of a comprehensive census of c...