نتایج جستجو برای: subcellular fractions
تعداد نتایج: 77371 فیلتر نتایج به سال:
We have analyzed Ca 2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter: :reporter gene activity, show that the Golgi CaZ+-ATPase, Pmrl, is the major Ca 2+ pump under normal growth conditions. In the absence of Pmrl, we show a massive, calcineurin-dependent ...
Maintaining proteostasis is thought to be a key factor in slowed aging. In several growth-restricted models of long-life, we have shown evidence of increased proteostatic mechanisms, suggesting that proteostasis may be a shared characteristic of slowed aging. The Snell dwarf mouse is generated through the mutation of the Pit-1 locus causing reductions in multiple hormonal growth factors and mTO...
Two nonmitochondrial forms of DNA polymerase are demonstrated in regenerating rat liver and Morris hepatomas 7777, 7800, and 7794A. The enzymes have been partially purified from subcellular fractions of these tissues. The purification procedure involves fractionation with ammonium sulfate and chromatography on diethylaminoethyl cellulose and on phosphocellulose. The enzymes have different physi...
Fresh anterior pituitary glands from beef and pig were separated by differential centrifugation into subcellular fractions. Nuclei and debris were obtained at 700 g for 15 minutes, secretory granules at 7000 g for 20 minutes, mitochondria at 34,000 g for 15 minutes, and microsomes at 78,000 g for 3 hours. Electron micrographs were taken of the individual fractions. Each fraction was analyzed fo...
Catalase (EC 1.11.1.6) oxidizes ethanol to acetaldehyde within the brain and variations in catalase activity may underlie some consequences of ethanol consumption. The goals of this study were to measure catalase activity in subcellular fractions from rat brain and to compare the levels of this enzyme in several important settings. In the first series of studies, levels of catalase were compare...
Pre-incubation of various subcellular fractions from rat brain caused a significant decrease in the phosphorylation of individual polypeptides. The rate and extent of this loss of labelling was not uniform and polypeptides whose phosphorylation was independent of cyclic AMP were primarily affected, whereas substrate availability remained unaltered. It is recommended that pre-incubation effects ...
The effect of phenobarbital on activities of aldehyde dehydrogenases in the subcellular fractions was studied in vivo in rats of three strains. Regarding the responses of the mitochondrial and microsomal enzymes to phenobarbital treatment no difference among the strains was observed. Regarding the responses of the supernatant enzyme remarkable strain differences were observed. In rats of the Lo...
Inhibition of simian virus 40 (SV40) DNA synthesis prevents late but not early viral gene expression in infected cells. To test whether the late SV40 template specificity is elicited by replicative intermediate DNA molecules (RI-DNA), we isolated subcellular fractions containing RI-DNA from SV-40-infected monkey cells and microinjected these preparations into various cell lines under conditions...
The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we ...
Using a radioimmunoassay, we have measured the level of calmodulin-dependent phosphatase (calcineurin) in various subcellular fractions from chick forebrain. Our results revealed high levels of the enzyme in the cytoplasm and microsomes. A considerable amount was also observed in synaptosomes, where it was found exclusively in the synaptoplasm, comprising 0.32% of the total synaptoplasmic prote...
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