A-factor is a potent pleiotropic effector produced by Streptomyces griseus and is essential for streptomycin production and spore formation in this organism. Its production is widely distributed among various actinomycetes including Streptomyces coelicolor A3(2). Genetic analysis of A-factor production was carried out with S. coelicolor A3(2), and two closely linked loci for A-factor mutations ...

2-Chloroethylphosphonic acid (CEPA) affected both the growth of and antibiotic production in Streptomyces aureofaciens, S. griseus, S. antibioticus, and Penicillium citrinum. Streptomyces strains seemed to be more sensitive to the presence of CEPA in the medium than did the fungus. A decrease in both growth and antibiotic production was observed with a concomitant increase in the concentration ...

Protease A and protease B are extracellular proteins which are secreted by Streptomyces griseus. The genes encoding protease A (sprA) and protease B (sprB) were isolated from an S. griseus genomic library by using a synthetic oligonucleotide probe. Fragments containing sprA and sprB were characterized by hybridization and demonstration of proteolytic activity in Streptomyces lividans. Each DNA ...

The utilization of various carbohydrates by species of streptomyces has been suggested by Pridham and Gottlieb (1948), Kurosawa (1951), and Benedict et al. (1955) as an aid in differentiation of species. While the latter investigators have reported that streptomycin-producing strains of Streptomyces griseus will grow on synthetic agar media containing as sole carbon source mannitol, xylose, fru...

Indole-substituted oxazolidinones, oxazolones, pyrrolidinone, imidazolidinone and imidazolones were synthesized. Their inhibitory potencies towards protein kinase C and protein kinase A were determined and their in vitro activities against Streptomyces chartreusis, Streptomyces griseus, Bacillus cereus, Candida albicans and Escherichia coli were examined. The inhibition of Streptomyces sporulat...

Antibiotic production and cell differentiation in Streptomyces is stimulated by micromolar levels of Cu(2+) . Here, we knocked out the Sco1/SenC family copper chaperone (ScoC) encoded in the conserved gene cluster 'sco' (the S treptomycescopper utilization) in Streptomyces coelicolor A3(2) and S. griseus. It is known that the Sco1/SenC family incorporates Cu(2+) into the active centre of cytoc...

Although fundamental problems relating to phage multiplication have been attacked with various genetic, biochemical, and morphologic methods, important questions still remain to be solved especially concerning gene and chromosome replications and DNA synthesis in phages. Recently phages which attack species of Streptomyces have become the subject of study by many workers because of the industri...

Although fundamental problems relating to phage multiplication have been attacked with various genetic, biochemical, and morphologic methods, important questions still remain to be solved especially concerning gene and chromosome replications and DNA synthesis in phages. Recently phages which attack species of Streptomyces have become the subject of study by many workers because of the industri...

The phage 514-3 of Streptomyces griseus described in the preceding paper (Gilmour et al., 1959) has from the time of its isolation from soil, showed a high infectivity for a large number of diverse Streptomyces species. The application of pure-lining procedures involving transfers from plaques and continued propagation on the homologous host S. griseus strain 3475 has not altered the observed b...