Sterols are essential lipid components of eukaryotic membranes. Here we summarize recent advances in understanding how sterols are transported between different membranes. Baker's yeast is a particularly attractive organism to dissect this lipid transport pathway, because cells can synthesize their own major sterol, ergosterol, in the membrane of the endoplasmic reticulum from where it is then ...

Sterols are important not only for structural components of eukaryotic cell membranes but also for biosynthetic precursors of steroid hormones. In plants, the diverse functions of sterol-derived brassinosteroids (BRs) in growth and development have been investigated rigorously, yet little is known about the regulatory roles of other phytosterols. Recent analysis of Arabidopsis fackel (fk) mutan...

A comparison has been made in vivo between IS+)-3hydroxy[3-Wlbutyrate, o(-)-3-hydroxy[3-“‘Clbutyrate, [3Wlacetoacetate, and n-[Z-Wlglucose for sterol and fatty acid synthesis and respiration in the B-day-old suckling rat. (a) Sterols and fatty acids in spinal cord, brain, and skin were preferentially labeled by these metabolites over sterols and fatty acids in the liver and kidneys. (b) More la...

OBJECTIVES The purpose of this research was to determine whether serum plant sterol levels are associated with those in atheromatous plaque. BACKGROUND Cholesterol of low-density lipoprotein (LDL) particles contributes to atheromatous plaque formation; LDL also contains most serum non-cholesterol sterols, including plant sterols. The role of plant sterols in atheromatous plaque formation is o...

Primary rat hepatocyte culture cells were used to study the acute regulation of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity in response to 25-hydroxycholesterol, 3 beta,5 alpha,6 beta-cholestantriol, and mevalonolactone. All three effectors caused a rapid suppression of HMG-CoA reductase activity. 25-Hydroxycholesterol also caused an increase in the ratio of newly synthes...

Sterols which have been identified in a transplantable preputial gland tumor are lanosterol, 24,25-dihydrolanosterol, 4cr-methylAs-cholestenol, A’-cholestenol, 7-dehydrocholesterol, and cholesterol (preceding paper (2)). Arranged in the above sequence, they suggest a possible pathway for the conversion of lanosterol to cholesterol. The studies presented in this report of acetate incorporation i...

A comparison has been made in vivo between IS+)-3hydroxy[3-Wlbutyrate, o(-)-3-hydroxy[3-“‘Clbutyrate, [3Wlacetoacetate, and n-[Z-Wlglucose for sterol and fatty acid synthesis and respiration in the B-day-old suckling rat. (a) Sterols and fatty acids in spinal cord, brain, and skin were preferentially labeled by these metabolites over sterols and fatty acids in the liver and kidneys. (b) More la...

Sterols, triterpenes, volatiles, polar and other constituents in aerial parts of Carthamus lanatus were analyzed by gas chromatography-mass spectrometry. Over 90 compounds were identified most of them new for the species. Sitosterol and stigmasterol were the most abundant of 10 sterols identified in the sterol fraction. Taraxasterol, alpha- and beta-amyrine prevailed in the triterpene fraction....

Mixed soybean sterols (SO per cent sitosterols) were fed to rats simultaneously with cholesterol in acute and chronic experiments. In acute experiments, concurrent feeding of a test dose of soybean sterols and cholesterol did not diminish the usual fraction of cholesterol absorption into the thoracic duct lymph. In chronic experiments the addition of 2 or 10 per cent soybean sterols to a diet w...