Fatty acyl esters of phytosterols are a major form of sterol conjugates distributed in many parts of plants. In this study we report an Arabidopsis (Arabidopsis thaliana) gene, AtSAT1 (At3g51970), which encodes for a novel sterol O-acyltransferase. When expressed in yeast (Saccharomyces cerevisiae), AtSAT1 mediated production of sterol esters enriched with lanosterol. Enzyme property assessment...

The effects of membrane sterol level on the susceptibility of LM cell plasma membranes to exogenous phospholipases A2 has been investigated. Isolated plasma membranes, containing normal or decreased sterol content, were prepared from mutant LM cell sterol auxotrophs. beta-Bungarotoxin-catalyzed hydrolysis of both endogenous phospholipids and phospholipids introduced into the membranes with beef...

Issue: The moisture content of a fill material will affect its compaction characteristics and needs to be evaluated during quality control of the fill operation. When the fill is drier than its optimum moisture content, any moisture in the fill serves as a lubricant and helps the relative movement of the particles when densifying under vibratory compaction. When the fill is wetter than its opti...

In generating textual summaries of data, the content determination problem is even more complicated when summarizing time-varying data, such as in weather or stockmarket report generation. As well as the maximum, minimum and mean, what is of interest is the behaviour of the variable over time; e.g. dramatic changes, trends and degree of variability in the data. For example, in the graph of temp...

RATIONALE Human atherosclerotic plaques contain large numbers of cells deprived of O(2). In murine atherosclerosis, because the plaques are small, it is controversial whether hypoxia can occur. OBJECTIVE To examine if murine plaques contain hypoxic cells, and whether hypoxia regulates changes in cellular lipid metabolism and gene expression in macrophages. METHODS AND RESULTS Aortic plaques...

Diethylstilbestrol (DES) was injected in doses ranging from 600 micro g to 0.4 micrograms/kg body weight into mature male rats over a 3 wk period. Profound effects on skin morphology and on sterol content of skin were noted. The sebaceous glands atrophied and the epidermis lost granularity. The concentrations of all skin sterols, with the exception of cholesterol, were reduced. At a dose level ...

ACAT1 (acyl-CoA:cholesterol acyltransferase 1) is thought to have two distinct sterol-binding sites: a substrate-binding site and an allosteric-activator site. In the present work, we investigated the structural features of various sterols as substrates and/or activators in vitro. The results show that without cholesterol, the plant sterol sitosterol is a poor substrate for ACAT. In the presenc...

Issue: The moisture content of a fill material will affect its compaction characteristics and needs to be evaluated during quality control of the fill operation. When the fill is drier than its optimum moisture content, any moisture in the fill serves as a lubricant and helps the relative movement of the particles when densifying under vibratory compaction. When the fill is wetter than its opti...

The sterol and fatty acid compositions of four amphotericin B-resistant and of two amphotericin B-susceptible Candida lusitaniae clinical isolates were determined. A flow cytofluorometric susceptibility test (FCST) with a membrane potential-sensitive cationic dye was used as a complement to the conventional method for selecting the isolates. Compared to susceptible isolates, resistant ones show...