نتایج جستجو برای: stabilityd phenylglycine aminotransferase
تعداد نتایج: 15535 فیلتر نتایج به سال:
backgrounds normal or elevated values of serum alanine aminotransferase level (alt) vary in different studies mostly related to characteristics of reference population including age, gender, body mass index, nonalcoholic fatty liver disease (nafld), and metabolic syndrome prevalence. objectives to measure upper normal limit (unl) for serum alt in an apparently healthy iranian old population (wh...
N-Carbamoylsarcosine amidohydrolase, a novel enzyme involved in the microbial degradation of creatinine in Pseudomonas putida 77, was purified 27-fold to homogeneity with a 63% overall recovery through simple purification procedures including successive ammonium sulfate fractionation, DEAE-cellulose chromatography, and crystallization. The relative molecular mass of the native enzyme estimated ...
Alpha-amino acid ester hydrolase (EC 3.1.1.43, AEH) is a promising biocatalyst for the production of semi-synthetic β-lactam antibiotics, penicillins and cephalosporins. The AEH gene from Xanthomonas rubrilineans (XrAEH) was recently cloned in this laboratory. The three-dimensional structure of XrAEH was simulated using the homology modeling method for rational design experiments. The analysis ...
A new brush-type HPLC chiral stationary phase (CSP) comprising π-acidic N-(3,5-dinitrobenzoyl)-D-α-phenylglycine chiral units bound to the quinoxaline units of modified γ-aminopropyl silica gel by a 1,2-diaminoethane spacer has been prepared by solid-phase synthesis. To test the CSP attempts were made to separate the enantiomers of twenty-two racemates with different functional groups and the r...
introduction: to determine an indicator for triclabendazole (tcbz) efficacy, alanine aminotransferase (alt) activity in fasciola hepatica (iranian isolate) parasite in presence and absence of tcbz was evaluated by an in vitro cultivation method. also, alt enzyme activity between none and parasitized-infected sheep liver tissues was assessed. method: the sheep livers were collected and transfer...
Penicillin acylase of Escherichia coli catalyses the hydrolysis and synthesis of beta-lactam antibiotics. To study the role of hydrophobic residues in these reactions, we have mutated three active-site phenylalanines. Mutation of alphaF146, betaF24 and betaF57 to Tyr, Trp, Ala or Leu yielded mutants that were still capable of hydrolysing the chromogenic substrate 2-nitro-5-[(phenylacetyl)amino]...
aim: the healthy ranges for serum alanine aminotransferase (alt) levels are less well studied. the aim of this study was to define the upper limit of normal (uln) for serum alt levels, and to assess factors associated with serum alt activity in apparently healthy blood donors. methods: a total of 1939 blood donors were included. alt measurements were performed for all cases using the same labor...
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