Cotton is the most important textile plant in the world and is one of the most important crops for the production of oilseed. Because of its worldwide economic importance, new cultivars are constantly being released in the world and consequently in the Greek market, as Greece is the largest producer in Europe. We used simple sequence repeat (SSR) markers for the identification and the phylogene...

PREMISE OF THE STUDY Bombax ceiba (Malvaceae), commonly known as silk cotton tree, is a multipurpose tree species of tropical forests. Novel expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized for the species using transcriptome analysis. METHODS AND RESULTS A total of 33 new EST-SSR markers were developed for B. ceiba, of which 13 showed polymorph...

due to the complex assessment of young walnut cultivars (juglansregia l.) based on morphological traits, advance molecular tools have provided a new prospect for cultivar identification and dna fingerprinting. in this study, specific molecular keys were identified for 5 iranian walnut cultivars (juglansregia l.) using 30 ssr markers. the results showed that 5 ssr markers produced polymorphic ba...

BACKGROUND Research into the protection of rare and endangered plant species involves genetic analyses to determine their genetic variation and genetic structure. Various categories of genetic markers are used for this purpose. Microsatellites, also known as simple sequence repeats (SSR), are the most popular category of markers in population genetics research. In most cases, microsatellites ac...

Sequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify 40 introns, 24 exons, and 23 promoter regions within 54 maize genes. We survey...

Blast, Pyricularia grisea (Cooke) Sacc., is one of the most destructive diseases of rice worldwide and canresult in significant reductions in yield. The use of resistant cultivars is the most economical and effectiveway of controlling rice blast. A variety of DNA markers, including plant defense-related candidategene markers are available for genetic characterization and molec...

We developed 22 SSRs by using 3 approaches, RAHM (random amplified hybridization microsatellites), 5’ anchored PCR methods and an enriched genomic library. Seventy of 22 SSRs could be successfully amplified for Pyrus spp., which showed highly polymorphic. Sixty Asian pear accessions from 6 Pyrus species were genetically identified by 9 SSR markers with a total of 133 putative alleles. The SSR m...

Despite several efforts in the last decade toward development of simple sequence repeat (SSR) markers in peanut, there is still a need for more markers for conducting different genetic and breeding studies. With the effort of the International Peanut Genome Initiative, the availability of reference genome for both the diploid progenitors of cultivated peanut allowed us to identify 135,529 and 1...

We genotyped strawberry cultivars by two newly selected and two previously reported SSR markers. All four markers produced interpretable electropherograms from 75 accessions consisting of 72 Fragaria × ananassa cultivars or lines and three octoploid Fragaria species accessions. These SSR markers were highly polymorphic; in particular, one of the newly developed markers, FxaHGA02P13, was capable...