A method was established for isolating antigen-specific murine helper T cells by selective binding to antigen-pulsed macrophage (Mphi) monolayers. Sheep erythrocyte (SRBC)-primed T cells, which remained strongly adherent to SRBC-pulsed syngeneic Mphi after 20 h in culture, were markedly enriched for helper activity when tested in the in vitro antitrinitrophenol (TNP) response to TNP-SRBC. Succe...

The effects of dietary high levels of heat treated locally produced flaxseeds on body weight gain, and immune response were evaluated using local Saudi chicken during the pre-laying period (17-23 wk). Diets were formulated included locally produced heat treated flaxseed roasted at 60 ̊C for 5 minutes to have 5 dietary levels 0, 10, 20, 30, and 40 % of flaxseed. Each diet was fed to 50 experiment...

Staphylococcal peptidoglycan (PG) possesses in vivo immunodulating activity and is a B-cell mitogen in mice. The effect of PG on in vitro immune response of mouse splenocytes to sheep erythrocytes (SRBC) was studied, as well as the relationships between in vivo and in vitro adjuvant, immunosuppressive, and mitogenic activities of PG in terms of dose response, time kinetics, and physical state. ...

Immunocompetence of the 2001 Ross 308 broiler strain and the 1957 Athens Canadian Randombred Control (ACRBC) strain was compared when they were given diets representative of those that were being used in 1957 and 2001. Antibody response against SRBC, in vivo lymphoproliferation against Phytohemagglutinin-P (PHA-P), and inflammatory and phagocytic responses of the macrophages were measured. The ...

We have investigated the ability of lymphocytes from normal subjects and patients with autoimmune thyroid diseases to respond to a thyroidal antigen (human thyroglobulin, hTG) and a non-thyroidal antigen (Keyhole limpet hemocyanin, KLH) in vitro, using a hapten (trinitrophenol, TNP)-carrier system. This system is based on the concept that the T helper cells which respond to hTG or KLH should st...

Daily gastric intubation of lipopolysaccharide (LPS)-responsive C3H/HeN, BALB/c, and Swiss mice with SRBC for 2 wk resulted in oral tolerance, whereas similarly treated LPS-nonresponsive C3H/HeJ mice gave splenic anti-SRBC PFC responses, including the IgA isotype, after systemic challenge with antigen. Oral tolerance in LPS-responsive C3H/HeN mice was due to T suppressor (Ts) cells because sign...

Phosphodiesterase (PDE) inhibitors can regulate the activity of immune cells by increasing intracellular levels of cyclic nucleotides. The aim of this study was to determine the effects of milrinone, a selective PDE3 inhibitor, sildenafil, a selective PDE5 inhibitor, and aminophylline, a nonselective PDE inhibitor, on lymphocyte subsets and humoral immune response in mice when administered in v...

OBJECTIVES: The effect of a single immunization with sheep red blood cells (SRBC) on the nocturnal NAT activity in the pineal gland and serum anti-SRBC agglutinin titer was examined in the young, sexually immature ASTRA S chickens. METHODS: 3-, 4-, and 5-week-old birds of both sexes, hatched in long (Spring) and short (Autumn) day, were housed from hatching in controlled light (L:D = 12:12) and...

OBJECTIVE To measure levels of IgG antibodies against structurally related synthetic peptides of HLA-DRB1*0404, type XI collagen, and Proteus mirabilis in patients with rheumatoid arthritis (RA) and HLA-B*2705 and Klebsiella pneumoniae in patients with ankylosing spondylitis (AS), and to determine whether sera from RA and AS patients are cytotoxic for sheep red blood cells (SRBC) coated with HL...

Concanavalin A, a nonspecific polyclonal activator of T lymphocytes, activates Lyl and Ly23 subclasses to the same degree. After activation, the Ly23 subclass, but not the Lyl subclass, has the following properties: (a) Suppression of the antibody response to sheep erythrocytes (SRBC) in vitro. (b) Production of a soluble factor that suppresses the anti-SRBC response in vitro. (c) Suppression o...