In an earlier study, 51% of total renal nuclear content of 3H-aldosterone was recovered bound to nuclear proteins readily extractable with Tris-3 fll~ CaC12. Modifications iu the methods used to prepare renal nuclear fractions resulted in the isolation of 3H-aldosterone bound to chromatin to the extent of 55% of the total nuclear content of the steroid. The chromatin binding system was stereosp...

AH-Aldosterone-ma :romolecular complexes were isolated from the nuclear and :ytosol fractions of rat kidneys by precipitation with (NHS&iOc and by chromatography on Sephadex G-50 and G-75. l’hese complexes were recovered after injection of 3H-aldostN crone into adrenalectomized rats and after incubation of la bel’ed steroid with renal nuclear extracts and cytosol fract. ons in vitro. The nuclea...

It was Raynaud and his co-workers") who in 1952 published at first their observations on the diuretic effect of heparin. The edema in a woman with congestive heart failure disappeared after the administration of heparin. Engelberg14) observed a similar effect in a patient with Kimmelstiel-Wilson syn drome. In the following years French and Belgian authors,31,22,32,13,7,8) reported on their obse...

The possibility that mineralocorticoids have a direct influence on renal Na-K ATPase activity has been the focus of intense research effort and some controversy for a number of years. Early studies were hindered by an inability to differentiate between possible glucocorticoid vs. mineralocorticoid effects on this enzyme within the multitude of cells that comprise the heterogeneous mammalian nep...

In humans, spironolactone and mespirenone are well known antimineralocorticoids without C-11beta substituents. These compounds display antagonist properties by acting through the human androgen receptor (hAR). In contrast, we demonstrate here that synthetic mineralocorticoid antagonists bearing hydrophobic C-11beta substituents and C-17gamma-lactone are potent hAR agonists in vitro. The three-d...

This study was designed to examine the effect of adrenal steroid hormones on the response of the toad bladder to vasopressin. Aldosterone enhanced the short-circuit current response, the osmotic water flow response, and the urea permeability response to vasopressin. Since aldosterone also enhanced the short-circuit current response and the osmotic water flow response to adenosine 3',5'-monophos...

The title compounds, rabdosinate and rabdosin B, were isolated from the leaves of Isodon japonica, and characterized by IR-NMR spectroscopy. The molecular geometry, vibrational frequencies and gauge including atomic orbital (GIAO-13C) chemical shift values of the title compounds have been calculated by using DFT/B3LYP method with 6-311++G(d,p) basis set. In addition, obtained results were relat...

In this paper, a new nano-size ionic liquid (nIL) has been synthesized from D-phenylalanine and oxalic acid (D-Phe-OX) via a simple procedure. The obtained nIL has been characterized by the fourier transform infrared spectroscopy (FT-IR), energy-dispersive X-ray spectroscopy (EDAX), thermogravimetric analysis (TGA), nuclear magnetic resonance spectroscopy (1</sup...