AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work's aims are AMPK identification, intracellular localization, and their role in human spermatozoa function. Semen was obtained from healthy human donors. Sperm AMPK and phospho-Th...

Cryopreservation of human spermatozoa free from cryoprotectant can avoid toxicity caused by highly concentrated cryoprotectant and a series of specific carriers have been previously explored, except for PDMS chip. Our study is aimed at exploring a novel device for ultra-rapid cryopreservation of small numbers of spermatozoa without cryoprotectant based on polydimethylsiloxane (PDMS) chips. Sper...

To date, cultivated male Japanese eels have not matured sexually in captivity under normal conditions. However, spermatogenesis and spermiation can be induced by the injection of gonadotropins. During this study, most males spermiated after the fifth or sixth weekly injection of human chorionic gonadotropin (HCG; 1 IU/g BW/wk) and the milt weight gradually increased as the number of injections ...

A series of protease inhibitors were tested on the motility of human, rat, bull, and rabbit demembranated reactivated spermatozoa. Some inhibitors, including aprotinin, boc-gln-leu-lys-H, and D-phe-pro-arg-H, could inhibit motility as well as prevent initiation of motility. In general, with the exception of aprotinin, protease inhibitors were more potent in preventing the initiation of movement...

introduction: pyrethroids are important insecticides because of their high activity and low mammalian toxicity. some studies have demonstrated that these insecticides, especially compounds with an -cyano group, produce toxic effects on various biological systems. deltamethrin (dtm) is a type ii synthetic pyrethroid insecticide containing -cyano group and is used worldwide in agriculture, home...

platelet activating factor (paf) is a novel signaling phospholipids that in addition to platelet activation has many biological properties. the acrosome reaction, as an essential step in mammalian fertilization, can occur in response to several agents such as paf. therefore, the present study aimed to assess the effect of paf on the motility and acrosome reaction of ram spermatozoa. semen was c...

Abstract This study is the first to investigate effects of tebuconazole (TEB) on physiological functions bovine testicular cells and epididymal spermatozoa. Motility plasma membrane integrity spermatozoa exposed TEB (0.001–100 µM) were evaluated at different incubation times (0–6 h), while TEB-induced spermiotoxicity was assessed after 24 h in cell cultures. Testicular cells, obtained from pare...

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets determine effectiveness adding sucrose tris-egg yolk on sperm swamp buffaloes produced by with egg albumin method. Semen was collected once week for 12 consecutive weeks artificial vagina from three male at Indonesian Center Animal Research Develop...

To compare the reproductive performance of the wild and cultured stocks of beluga, Huso huso males, some spermatological parameters were measured including: sperm motility, sperm density, spermatocrit and also fertilization rate, hatching rate and larval growth and survival rates. The semen samples were sampled from 11 wild spawners and 12 cultured brooders. A half of each semen sample was allo...

BACKGROUND Sodium arsenite can impair male reproductive function by inducing oxidative stress. Silymarin is known as a potent antioxidant. OBJECTIVE This study was performed to investigate if silymarin can prevent the adverse effect of sodium arsenite on ram sperm viability, motility and mitochondrial membrane potential. MATERIALS AND METHODS Epidydimal spermatozoa obtained from ram were di...