Understanding the mechanisms by which the acrosome reaction is regulated is central to models of fertilization. This article reviews the relationship of the acrosome reaction detected in vitro to sperm function. Proteolytic enzymes in the acrosome digest through the zona pellucida allowing for sperm-oolemma fusion. When this process is impaired, either by lack of an acrosome or acrosomal dysfun...

casa (computer assisted semen analysis) systems are designed to assist andrologist labour. most available casa systems are not accurate or so expensive. therefore labours use manual methods to provide parameters. although some companies have achieved appropriate accuracy, they have not released their methods. so proposing methods in this area might be useful for groups who intend to design new ...

Boar semen cryopreservation remains a challenge due to the extension of cold shock damage. Thus, many alternatives have emerged to improve the quality of frozen-thawed boar sperm. Although the use of seminal plasma arising from boar sperm-rich fraction (SP-SRF) has shown good efficacy; however, the majority of actual sperm evaluation techniques include a single or dual sperm parameter analysis,...

BACKGROUND The value of varicocele repair and the latter's impact on semen parameters are still subject to debate. METHODS We analyse changes over time in initially abnormal sperm parameters and serum concentrations of testosterone, FSH and inhibin B after embolization treatment of males with high-grade varicocele. From 2007 to 2012, we recruited 47 male infertile patients with clinically vis...

BACKGROUND Patients with poor semen quality show increased sperm disomy and diploidy rates. Oligozoospermia and teratozoospermia are known to influence sperm aneuploidy, but there is still a debate about whether aneuploidies are associated with reduced motility. METHODS Ejaculates from a large group of patients were examined by light microscopy to evaluate sperm concentration, motility and mo...

BACKGROUND This study takes an in depth look at embryonic development, implantation, pregnancy and live birth rates with frozen epididymal and testicular sperm from obstructed (OA) and non-obstructed (NOA) patients. METHODS Paternal effect of sperm source on zygote formation, embryonic cleavage, and genomic activation were examined. Additional outcome parameters monitored were clinical pregna...

Several sperm parameters have been employed as useful tools to evaluate fish fertility. Within teleosts, approximately 3% of fish species are known to be viviparous. The Order Cyprinodontiformes includes several species with internal fertilization, and within this group most of the studies about sperm quality have been mainly focused on the Poeciliidae family. The livebearing fish Jenynsia mult...

The fertilizing ability of stallion semen was analyzed using fresh and frozen samples, obtained before (June-July) or during (October-November) the breeding season. Thirty ejaculates obtained from 4 stallions were used. The analysis comprises routine seminogram; ATP concentration (Comhaire et al., 1983); subjective and objective motility and sperm velocity (Makler, 1980). Freezing was done foll...

Background Sperm motility is known as an effective parameter in male fertility and it depends on energy consumption. Low level LASER irradiation could increases energy supply to the cell by producing of adenosine triphosphate (ATP). The purpose of this study is to evaluate how the low level LASER irradiation affects the human sperm motility. MaterialsAndMethods Fresh human semen specimens of as...

OBJECTIVE The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. METHODS We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guideli...