Intracytoplasmic sperm injection has revolutionised the management of male infertility. We report two cases that demonstrate the successful application of this technology in Nigeria in the management of both oligospermia and azoospermia. The first case relates to the treatment of a 31-year-old woman who required intracytoplasmic sperm injection of her husband's sperm for the treatment of both t...

BACKGROUND For human ICSI, sperm are normally immobilized immediately prior to injection. However, there are some situations when only sperm of questionable viability are available. There are few evaluations of fertilization or developmental problems in human or animal models using sperm having known intervals between immobilization and injection. METHODS Immobilized human sperm were maintain...

Background: Sperm mediated gene transfer was approved for producing transgenic animals. There are many studies which reported different success rate by this procedure. Failure in this project mostly is returned to transfection and stability of transferred genes. The other problem that may contribute in project failure may return to the testicular damages which occur at the time of transfection,...

PURPOSE We evaluated the retrieval rates and reproductive outcomes of percutaneous sperm retrieval according to the cause of obstructive azoospermia. MATERIALS AND METHODS We retrospectively studied the records of 146 men with obstructive azoospermia who underwent sperm retrieval for intracytoplasmic sperm injection. Patients were grouped by the cause of obstruction, including 32 with congeni...

Received on: April 28, 2009 – Accepted on: Oct 15, 2009 aBstRact Azoospermia is defined as the absence of mature spermatozoa in semen analysis after centrifugation. The causes of azoospermia may be related to spermatogenesis defects and classified as nonobstructive azoospermia, or to the obstruction of the efferent ducts of the genital tract and classified as obstructive azoospermia. Before the...

Injection of eggs of various species with an extract of sperm cytoplasm stimulates intracellular Ca(2+) release that is spatially and temporally like that occurring at fertilization, suggesting that Ca(2+) release at fertilization may be initiated by a soluble factor from the sperm. Here we investigate whether the signalling pathway that leads to Ca(2+) release in response to sperm extract inje...

Bulls treated with ethylene dibromide (EDB), either by oral administration of ten doses of 4 mg/kg body weight on alternate days or by a single intraperitoneal injection of 110 -2 mg of this compound into the fluid surrounding each testis, exhibited sperm malformations up to total disintegration of the sperm cells. The malformations appeared in the ejaculates collected about two weeks after the...

We assessed the rates of sperm retrieval and intracytoplasmic sperm injection outcomes, including the neonatal profile of infants conceived, in men with testicular failure. Three-hundred and sixty-five men with testicular failure who underwent micro-dissection testicular sperm extraction were included in this study. We compared their outcomes with 40 men with testicular failure who used donor s...

Submit Manuscript | http://medcraveonline.com Abbreviations: WHO: World Health Organization; ROS: Reactive Oxygen Species; PS: Phosphatidylserine; EPS: Externalization of Phosphatidylserine; ART: Assisted Reproduction Techniques; IUI: Intra-Uterine Insemination; IVF: In Vitro Fertilization; ICSI: Intracytoplasmic Sperm Injection; PICSI: Physiologic Intracytoplasmic Sperm Injection; SU: Swim-Up;...