A primary standard for positron-emitters in gas has been developed. The method involves internal gas proportional counting and the use of the PENELOPE Monte Carlo code to determine corrections for counting losses. The development work was carried out using (11)C, although the method can be applied to other positron emitters. The results were compared with measurements of (11)C (in solution) car...

Transwell Boyden chamber based migration/invasion assay is a simple and extensively used approach for the characterization of cell motility in vitro. Cell motility is quantified by counting the number of cells that pass through the filter membrane. The counting is usually performed manually, which is laborious and error prone. We have therefore developed CELLCOUNTER, an application that is capa...

Busulfan is an anticancer drug, which causes the apoptosis germ cells and azoospermia in humans and animals. Abnormal morphology of spermatozoa related to the male infertility. The sperm morphology is evaluation of sperm size, shape and appearance characteristics should be assessed by carefully observing a stained sperm sample under the microscope. Evaluation of sperm morphology has been consid...

Objective: To determine the impact of Covid-19 vaccines on sperm quality. Study Design: Case control study Place and Duration Study: Department Diabetes & Endocrinology, Chandka Medical College Hospital Larkana from 1st July 2022 to 31st December 2022. Methodology: Patients were enrolled as 50 those who had PCR confirmed Covid 19 history never got Covid-19. On this basis cases a placed in g...

Various counting methods have been described and reported for process control of leuco-epleted blood components. The recent production of high-efficiency leucocyte removal filters intensifies the need for sensitivity in determining the ever lower residual concentration of white cells (WBCs) in filtered units. In order to assess which method was the most efficient and feasible in the laboratory ...

The large size of primate brains is an impediment to obtaining high-resolution cell number maps of the cortex in humans and non-human primates. We present a rapid, flow cytometry-based cell counting method that can be used to estimate cell numbers from homogenized brain tissue samples comprising the entire cortical sheet. The new method, called the flow fractionator, is based on the isotropic f...

1 Department of Biology, Southeast Missouri State University, Cape Girardeau, MO 63701, USA. 2 CNRS-UMR5175 CEFE, Centre d’Ecologie Functionnelle et Evolutive, 1919 route de mende, 34293 Montpellier cedex 5. 3 College of Sciences, The University of Findlay, Findlay, Ohio 45840, USA. 4 Department of Biological Sciences, Southeastern Louisiana University, Hammond, LA 70402, USA. * Corresponding a...

The aim of this study was (1) to analy�e fresh intact or differently influenced rabbit sperm samples, using Annexin V–binding method for the detection of membrane phosphatidylserine translocation, and (2) to estimate obtained results in relation to the conceptional rate of rabbit females fertili�ed with semen from different groups. Following sample groups were designed: 1. freshly collected sem...

A simple method is described whereby measurement of neutrophil chemiluminescence was performed at variable reaction temperatures even though the scintillation counting chamber was refrigerated.