To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatographic separat...

PURPOSE This study was designed to develop and validate two simple, rapid, and economical UV-spectrophotometric and the first-order derivative methods using the area under curve method for estimation of diacerein in bulk and in capsule formulation. MATERIALS AND METHODS In this study, hydrotrophic solution of 8 M urea and 0.5 M potassium citrate were employed as the solubilizing agent to solu...

gabapentin is an anticonvulsant widely used in the treatment of epilepsy.an accurate and validated spectrophotometric method was developed as an alternative method to determine gabapentin in pharmaceutical products. this is a simple, accurate, precise, selective and visible spectrophotometric method. the method is based on the measurement of drug absorbance as a result of yellow solution format...

UNLABELLED In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatograp...

In this study, derivative UV spectrophotometric methods were developed for the analysis of diflunisal, which is used as a nonsteroidal anti-inflammatory drug. Analysis wavelengths of diflunisal for the first and second order derivative UV spectrophotometric methods were obtained as 235.6 nm and 228.8 nm, respectively. The linearity ranges were found as 1.0-25.0 μg mL-1 for the first order and 1...

A spectrophotometric method for the determination of chlorine,bromine or iodine ion by using1,5-bis(6-methy1-4-pyrimidyl)carbazone has been established.The ranges of determination were0.355-3.55ppm of Cl-,0.799-7.99ppm of Br and1.27-12.7ppm of F.The proposed method could be applied to the analyses of various halogen-containing organic compounds, including sulfur-containing compounds.Eleven comm...

A simple, precise economical and fast visible spectrophotometric method has been developed for the determination of Itopride hydrochloride in tablet dosage form. Developed is based on formation of extractable colored complex of drug with coloring agent mordant blue 3. A wavelength maximum was found to 471.0 nm. Linearity was observed 30-70 mg /mL. The proposed method describes the determination...

A simple and derivative spectrophotometric method has been developed for the determination of mercury (II) using Diacetylmonoxime benzoylhydrazone (DMBH). Mercury (II) forms an orange coloured water soluble complex with the reagent in basic medium (pH 10.5).The molar absorptivity and Sandell’s sensitivity of coloured species are 2.01 x 10 L.mol cm and 0.0099 μg/cm respectively. Beer’s law is ob...

A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 μg/ml. The relative standard devi...