Present study deals with two Spectrophotometric methods, Conventional Spectrophotometric Estimation and Area under Curve Method and a chromatographic method for estimation of Cefixime by using five different hydrotropic agents. These include Ammonium acetate (6M), Potassium acetate (5M), Potassium citrate (0.5 M), Sodium citrate (1.25 M) and Urea (8M). Area under curve method was based on measu...

Purpose: To improve the sensitivity and specificity of spectrophotometric 3-hydroxy-3-methylglutarylcoenzyme A (HMG-CoA) reductase activity assay. Methods: Spectrophotometric HMG-CoA reductase detection in male Wistar rat liver microsomes was optimized by applying different conditions, such as reaction buffer pH, NADPH and protein concentration, and preincubation and reaction times. The optimal...

The present work describes a new HPLC method and a new spectrophotometric method for the simultaneous determination of ethinyl estradiol (EE) and levonorgestrel (LNG) in their binary combination. In LC method, Nucleosil C8 column with a mobile phase composed of acetonitrile-water (90:10, v/v) with UV detection at 225 nm were used for the analysis of ethinyl estradiol-levonorgestrel combination....

New simple and sensitive spectrophotometric and fluorimetric methods have been developed and validated for the determination of fluoxetine hydrochloride (FLX) in its pharmaceutical formulations. The spectrophotometric method was based on the reaction of FLX with 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium (pH 11) to form an orange-colored product that was measured at 490 nm. The...

UNLABELLED In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatograp...

Spectrophotometric methods for the determination of total polyphenols and flavonoids are often used because they simple, sensitive, precise. Before using these methods, must be validated to ensure quality obtained data prove that method is suitable its purpose. The aim this study was validate spectrophotometric flavonoids. has been according polyphenol standards, which belong groups phenolic ac...