Computerization is necessary to build the database of chromatography coupled mass spectrometric analytical data. On the basis of this proteomic data we can identify the proteins as biomarkers which are expressing dissimilarity between healthy and disease condition. Affinity chromatography is one of the fastest liquid chromatographic methods for the separation and purification of biomolecules du...

The Major Urinary Proteins (MUPs) of the house mouse, Mus musculus domesticus, are 19 kDa beta-barrel lipocalins that are involved in chemical communication between individuals. Many of them are excreted in urine where they play multiple roles, including coding of owner identity and transport, and slow release of bound volatile pheromones. It has been shown that mass spectrometric analysis of i...

The progressive increase of invasive disease and reports of resistance among Aspergillus species emphasizes the need for reproducible antifungal susceptibility testing. Inoculum standardization is a crucial step in such procedures. The objective of this study was to develop a fast and precise method of evaluating the cell density of an Aspergillus spore suspension, as an alternative to spectrom...

Two prenylated indole alkaloids were isolated from the ethyl acetate extracts of a marine-derived fungus Penicillium sp. NH-SL and one of them exhibited potent cytotoxic activity against mouse hepa 1c1c7 cells. In order to detect other bioactive analogs, we used liquid chromatogram tandem mass spectrometry (LC-MS/MS) to analyze the mass spectrometric characteristics of the isolated compounds as...

Mass spectrometric studies of the vapours over solid lead oxide in a flow of gaseous arsenic and antimony trioxides were conducted. The following ions of the ternary oxides were detected: Pb3As2O6(+), Pb3AsO4(+), PbAs2O4(+), PbAsO2(+), PbSb2O4(+), and PbSbO2(+). The origin of these species produced by the ionisation and/or fragmentation of ternary gaseous oxides is discussed. The PbAs2O4 specie...

Modified urinary nucleosides are potentially invaluable in cancer diagnosis, as they reflect altered RNA turnovers. High-performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, tandem mass spectrometry, MS(n) analysis and accurate mass measurements in order to identify pyrimidine nucleosides purified from urine. Potential nucleosides were assessed by their eviden...

Mass spectrometric methods were used for the fi rst time to reveal the sequences of peptide orthologs from a large number of insects species, focussing on cockroaches. Sequence data of CAPA peptides (three periviscerokinins, pyrokinin) were obtained from single specimens, which led to the reconstruction of phylogenetic trees. The results are compared with the current grouping of the respective ...

The capabilities and applicability of the radiotracer technique for methodological studies in hydride generation and electrothermal atomic absorption spectrometry are discussed and demonstrated by using several selected examples. It is shown that, for studying chemical and physico-chemical aspects of atomic absorption spectrometric methods, the radiotracer technique is superior to other techniq...

This paper proposes new clustering criteria for distinguishing Saccharomyces cerevisiae (yeast) strains using their spectrometric signature. These criteria are introduced in an agglomerative hierarchical clustering context, and consist of: (a) minimizing the total volume of clusters, as given by their respective convex hulls; and, (b) minimizing the global variance in cluster directionality. Th...