Effects of different levels of cadmium (0.1, 1, 2, 4, 8, 16, 32, 64, 128, 256 mg/l of culture medium) were evaluated using gas production technique. In the glass vials used to measure gas production 30 mg of buffered rumen fluid (2:1 ratio of rumen fluid: buffer) poured in glass and cultured at 38.6 temperature. Volume and pressure of produced gas were measured with syringes and digital pressur...

Effects of different levels of cadmium (0.1, 1, 2, 4, 8, 16, 32, 64, 128, 256 mg/l of culture medium) were evaluated using gas production technique. In the glass vials used to measure gas production 30 mg of buffered rumen fluid (2:1 ratio of rumen fluid: buffer) poured in glass and cultured at 38.6 temperature. Volume and pressure of produced gas were measured with syringes and digital pressur...

Rumen, main compartment of the stomach ruminant herbivores, have a low redox potential and anaerobic ecosystem in where various microbial populations inhabit, such as bacteria, gut fungi (AGF), protozoa archaea. These groups symbiotic life whilist both synergetic releationship inhibition some activities could be observed when they are cultured mixture. Relatively higher enzyme AGF is well docum...

The rumen microbiome represents a complex microbial genetic web where bacteria, anaerobic rumen fungi (ARF), protozoa and archaea work in harmony contributing to the health and productivity of ruminants. We hypothesized that the rumen microbiome shifts as the dairy cow advances in lactations and these microbial changes may contribute to differences in productivity between primiparous (first lac...

Anaerobic fungi were isolated from rumen fluid of a domestic sheep (Ovis aries; a ruminant) and from faeces of five non-ruminants: African elephant (Loxodonta africana), black rhinoceros (Diceros bicornis), Indian rhinoceros (Rhinoceros unicornis), Indian elephant (Elephas maximus) and mara (Dolichotis patagonum). The anaerobic fungus isolated from the sheep was a Neocallimastix species and the...

DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of their physical nature and associated microorganisms. The aim of this study was therefore to asses...

A common screen for plant antimicrobial compounds consists of separating plant extracts by paper or thin-layer chromatography (PC or TLC), exposing the chromatograms to microbial suspensions (e.g. fungi or bacteria in broth or agar), allowing time for the microbes to grow in a humid environment, and visualizing zones with no microbial growth. The effectiveness of this screening method, known as...