Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

in this paper we investigate the effect of oil price shocks on stock market index in iran, by using of a structural var (svar) approach. we used four variables in the model namely kilian index, global oil supply, real oil price and real stock market index. the data are monthly and spanning the period 1997m10-2014m12. we identify the effect of four different shocks on stock market including oil ...

Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...

background: candida albicans (c. albicans) is a major cause of candidaemia in people with impaired immunity. blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. we established a real-time pcr assay for c. albicans detection in blood by lightcycler pcr and melting curve analysis. methods: five milliliter blood samples from healthy volunt...

background and purpose: invasive candidiasis (ic) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent ic progress is necessary. we established a real-time pcr assay on blood for the diagnosis an...

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...