نتایج جستجو برای: specific pcr
تعداد نتایج: 1175031 فیلتر نتایج به سال:
invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. an early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. recently, the polymerase chain reaction (pcr) has been used successfully in detecting specific dna of several pathogen. in this study, nested pcr was used to detect dna specific for a!.pergiflus s...
چکیده اسکیزوفرنی و اسکیزوافکتیو و بیماری دوقطبی، اختلالات رایج روانی با وراثت پذیری بالا و فنوتیپ متغییر هستند. ژن disc1 که بر روی بازوی بلند کروموزوم شماره 1 ، ناحیه 42 (یا 1q42) واقع است که دارای نقش مهمی در رشد نورون ها و پیام رسانی سلولی می باشد. هدف از این تحقیق، بررسی ارتباط سه پلی مورفیسم تک نوکلئوتیدی rs6675281، rs2255340 و rs2738864 با اختلال اسکیزوفرنی است و این پروژه برای اولین بار ...
objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...
Abstract Background and Objective: Human Cytomegalovirus (CMV) is an important cause of congenital viral infection that can lead to serious diseases and complications in infants. Application of rapid, sensitive, and specific HCMV detection methods is necessary for congenital infection detection. We aimed to optimize the use of PCR and ELISA for detection of HCMV in infants. Material and Methods...
Selecting specific primers is crucial for polymerase chain reaction (PCR). Nonspecific primers will bind to unintended genes and result in nonspecific amplicons. MFEprimer is a program for checking the specificity of PCR primers against the background DNA. In this chapter, we introduce: (1) the factors that affect the specificity of primers; (2) the principle of MFEprimer and its settings; (3) ...
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