نتایج جستجو برای: spa typing

تعداد نتایج: 26930  

Journal: :Journal of clinical microbiology 2013
Cara Nethercott Amanda N Mabbett Makrina Totsika Paul Peters Juan C Ortiz Graeme R Nimmo Geoffrey W Coombs Mark J Walker Mark A Schembri

Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gene microarrays are frequently used to classify S. aureus clinical isolates. Thi...

Journal: :iranian journal of microbiology 0
seyed-asghar havaei department of pathobiology, school of medicine, isfahan university of medical sciences, isfahan, iran. amir azimian department of pathobiology and anatomy, school of medicine, north khorasan university of medical sciences, bojnurd, iran. hosein fazeli department of pathobiology, school of medicine, isfahan university of medical sciences, isfahan, iran. mahmood naderi molecular biology and genetic engineering department, stem cell technology research center, tehran, iran. kiarash ghazvini department of microbiology, school of medicine, mashhad university of medical sciences, mashhad, iran. siamak mirab samiee food and drug laboratory research center, ministry of health and medical education, no. 408, emam khomeini ave., tehran, iran.

background and objectives: methicillin resistant staphylococcus aureus (mrsa) strains are divided into community associated (ca-) and hospital associated (ha-) mrsa. these strains vary in antimicrobial resistance and pathogenicity. s. aureus is one of the most common microorganisms in ocular infections. this study was aimed to determine antimicrobial resistance patterns and genetic characterist...

Journal: :Journal of clinical microbiology 2012
Matthew V N O'Sullivan Fei Zhou Vitali Sintchenko Gwendolyn L Gilbert

In settings of high methicillin-resistant Staphylococcus aureus (MRSA) prevalence, detection of nosocomial transmission events can be difficult without strain typing. Prospective typing of all MRSA isolates could potentially identify transmission in a timely fashion, making infection control responses to outbreaks more effective. We describe the development and evaluation of a novel 19-target b...

Background and purpose: The aims of this study were to evaluate the antibiotic resistance of Healthcare-associated Methicillin-resistant Staphylococcus aureus (HA-MRSA) and Community associated MRSA (CA-MRSA)-resistant strains of Staphylococcus aureus strains and to investigate the frequency of different types of spa typing, SCCmec I, II, III, IV, V, and type IV, among the strains of MRSA isol...

Journal: :Journal of clinical microbiology 2008
Alexander Mellmann Thomas Weniger Christoph Berssenbrügge Ursula Keckevoet Alexander W Friedrich Dag Harmsen Hajo Grundmann

We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).

Journal: :European journal of microbiology & immunology 2013
Israr Sabri Kamel Adwan Tamer A Essawi Mohammad A Farraj

Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates in three different Arab world countries (West Bank of Palestine, Jordan, and Iraq) was the aim of the study presented here. This is done on the basis of spa sequencing and staphylococcal cassette chromosome mec (SCCmec) typing. The majority (92%) of the spa-tested isolates belonged to spa type t932 and pos...

Journal: :The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 2015
Hala M Abou Shady Alaa Eldin A Bakr Mahmoud E Hashad Mohammad A Alzohairy

Epidemiological and molecular data on community acquired methicillin resistant Staphylococcus aureus (CA-MRSA) are still scarce in both Egypt and Saudi Arabia. There is almost no data regarding methicillin resistant Staphylococcus aureus (MRSA) prevalence in both countries. This study was conducted to investigate the prevalence and molecular epidemiology of S. aureus and MRSA nasal carriage amo...

2014
Yong Chen Zhengxiang Liu Libo Duo Jie Xiong Yanwen Gong Jiyong Yang Zhanke Wang Xuqin Wu Zhongyi Lu Xiangzhao Meng Jingya Zhao Changjian Zhang Fang Wang Yulong Zhang Mengqiang Zhang Li Han

Staphylococcus aureus belongs to one of the most common bacteria causing healthcare and community associated infections in China, but their molecular characterization has not been well studied. From May 2011 to June 2012, a total of 322 non-duplicate S. aureus isolates were consecutively collected from seven tertiary care hospitals in seven cities with distinct geographical locations in China, ...

Journal: :International journal of medical microbiology : IJMM 2013
Corinna Glasner Artur J Sabat Annette Dreisbach Anders R Larsen Alexander W Friedrich Robert L Skov Jan Maarten van Dijl

Methicillin-resistant Staphylococcus aureus (MRSA) represent a serious threat for public health worldwide. Of particular concern is the emergence of community-acquired MRSA, which is often difficult to distinguish from nosocomial MRSA due to a lack of suitable typing methods for early detection. For example, the USA300 pulsed-field gel electrophoresis (PFGE) pattern includes both the 'classical...

Journal: :Journal of clinical microbiology 2010
Olga Sakwinska Dominique S Blanc Catherine Lazor-Blanchet Martine Moreillon Marlyse Giddey Philippe Moreillon

We described the colonization dynamics of Staphylococcus aureus in a group of 266 healthy carriers over a period of approximately 1 year. We used precise genotyping methods, i.e., amplified fragment length polymorphism (AFLP), spa typing, and double-locus sequence typing (DLST), to detect changes in strain identity. Strain change took place rather rarely: out of 89 carriers who had initially be...

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