Liquid nitrogen freezing is recommended for long-term preservation of Leptospira serovars. However, there is no standard protocol to follow for this methodology. We herein report a simple procedure to preserve well-characterized Leptospira serovars unaltered for long-term storage in liquid nitrogen. Forty-three (43) leptospira strains, cryoprotected with 10% (v/v) glycerol were rapidly frozen i...

atmospheric nitrogen fixation as an exclusive phenomenon is accomplished by nitrogenase enzyme of prokaryotic microorganisms namely diazotrophs including bacteria, actinomycets and cyanobacteria. nitrogen fixation is accomplished as form of symbiotic, associative and free-living systems. in free-living systems nitrogen is fixed without the help of plants. azotobacter, the first diazotroph was d...

The preparation and electrical properties of high-temperature superconductor nanowire arrays are reported for the first time. YBa2Cu3O(7-delta) nanowires with widths as small as 10 nm (much smaller than the magnetic penetration depth) and lengths up to 200 microm are studied by four-point electrical measurements. All nanowires exhibit a superconducting transition above liquid nitrogen temperatu...

stan-2b ,16b-ylene)bis(1-methyl-piperidinium) dibromide [15500-66-0] is general anaesthesia used in surgery for muscle relaxation and as an aid to endotracheal intubation (Chart 1). Several methods of determination of this drug have been reported in the literature, including high performance liquid chromatography (HPLC), capillary gas chromatography using nitrogen sensitive detection, liquid ch...