somatic embryos of persian walnut were transformed with two strains of agrobacterium tumefaciens i.e. lba4404 and c58, and two plasmids, namely, pbi121 with nptii and gus genes for improving the transformation protocol, and p6u-ubi-fvti plasmid containing the hpt and fld genes. the transformation frequency was 10%. pcr and rt-pcr analysis proved the presence and expression of the genes. the tra...

plant regeneration was achieved in verbascum speciosum schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. two types of calli, embryogenic and non-embryogenic, were induced from mature embryo explants on murashige and skoog (ms) medium supplemented with different concentrations of benzyl adenine (ba) and α-naphthalene acetic acid (naa). in order to further ...

Germ cells normally differentiate in the context of encapsulating somatic cells. However, the mechanisms that set up the special relationship between germ cells and somatic support cells and the signals that mediate the crucial communications between the two cell types are poorly understood. We show that interactions between germ cells and somatic support cells in Drosophila depend on wild-type...

Somatic embryogenesis is an in vitro system employed for plant propagation and the study of embryo development. Nitrogen is essential for plant growth and development and, hence, the production of healthy embryos during somatic embryogenesis. Glutamine has been shown to increase plant biomass in many in vitro applications, including somatic embryogenesis. However, several aspects of nitrogen nu...

In mammals, somatic growth is rapid in early postnatal life but decelerates with age and eventually halts, thus determining the adult body size of the species. This growth deceleration, which reflects declining proliferation, occurs simultaneously in multiple organs yet appears not to be coordinated by a systemic mechanism. We, therefore, hypothesized that growth deceleration results from a gro...

Clonal production of loblolly pine (Pinus taeda L.) through somatic embryogenesis has great potential to meet the increasing industrial demands for highquality uniform raw materials. A major barrier to the commercialization of this technology is the low quality of resulting embryos. Twenty-five newly initiated loblolly pine genotypes were followed through the process of liquid culture establish...

In contrast to multicellular animals, plants lack a reserved germ line; their gametes are formed late in their growth cycles by differentiation of somatic meristematic cells. Typically, the somatic precursors of gametophytes have divided many times, potentially subjecting their genomes to multiple rounds of spontaneous or environmentally induced mutagenesis (Walbot, 1985). However, plants do no...

abstract to produce embryogenic callus and somatic embryo, whole flower explants were collected at two sampling stages of i, iii. to produce embryogenic callus and somatic embryo, ms medium supplemented with 5 and 10µm 2,4-d and 2µm bap were used. for embryo differentiation, ms medium with 0.5mg/l iba, ms medium without any plant growth regulators, ms medium with 2mg/l iba and 0.2mg/l bap, ms m...

Growth patterns of fish result from their energy allocation strategy, life stages and the environmental conditions in which they reside. Temperature, for example, can potentially drive size-dependent metabolic rates, then influence physiological functions such as food consumption leading to growth variation (Barneche & Allen, 2018; Madenjian et al., 2018). Previous studies suggest that common t...