Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog medium...

Somaclonal variation in plants regenerated by organogenesis from long-term cultured calluses of two diploid staminate genotypes of Asparagus officinalis cv. Argenteuil was characterized by plant phenotype, ploidy, meiotic behavior, pollen viability, fruit and seed set, and AFLP profiles. Phenotypic deviations from the donors were detected in foliage color, flower size, and cladode and flower mo...

Tissue culture studies of Celosia cristata were established from various explants and the effects of various hormones on morphogenesis of this species were examined. It was found that complete plant regeneration occurred at highest percentage on MS medium supplemented with 2.0 mg/L NAA and 1.5 mg/L BAP, with the best response showed by shoot explants. In vitro flowering was observed on MS basal...

The objective of this review is to summarize numerous studies on the use of the random amplified polymorphic DNA (RAPD) technique on rice, corn, wheat, sorghum, barley, rye, and oats to examine its feasibility and validity for assessment of genetic variation, population genetics, mapping, linkage and marker assisted selection, phylogenetic analysis, and the detection of somaclonal variation. Al...

turfgrasses are usually important groundcover plants in many landscapes. they occupy the lowest surface of the landscape, close to pollutant particles. so, they can be an attractive option for environmental remediation. today, high concentrations of hazardous chemicals such as lead are among the most serious environmental problems. in vitro selection of turfgrass accumulator or tolerant of toxi...

Hortic. bras., v. 22, n. 2, abr.-jun. 2004 C potato (Solanum tuberosum L.) is one of the most important vegetable crop of Brazil and the world’s fourth most important crop (Solomon-Blackbourn & Barker, 2001). In 2002, Brazil produced 3,126,411 t of potato with an average yield of 19,4 kg/ ha (IBGE, 2002). Potato is a tetraploid species and the use of its botanical seeds in commercial cultivatio...

Somaclonal variation has been observed in many plant species and is an alternative way to create variants and expand the germplasm pool. A large scale tissue culture experiment was conducted with St. Augustinegrass, an important turfgrass species for the southern USA, to induce somaclonal variation to enlarge the germplasm pool for breeding efforts. Using an improved protocol, approximately 790...