Organic solvents used for solubilization of the substrates/NCEs are known to affect the activity of cytochrome P450 enzymes. Further, this effect varies with the solvents used, the substrates and CYP450 isoforms in question. In the present study, we have investigated the effect of ten commonly used water miscible organic solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, acetone...

The photophysical properties of o-aminoacetophenone (o-AAP) in solution have been studied by using a femtosecond laserÈsingle photon counting system and time-resolved thermal lensing (TRTL) method. The Ñuorescence quantum yield and lifetime of o-AAP depend strongly on the nature of the solvent. In f (qf) nonpolar solvents, o-AAP gives very small values in n-hexane) and remarkably short f (Uf\ 2...

Three model, unrelated enzymes (yeast alcohol oxidase, mushroom polyphenol oxidase, and horse liver alcohol dehydrogenase) were found to be catalytically active in a variety of organic solvents. For all enzymes and solvents tested, the enzymatic activity greatly increased upon an increase in the water content in the solvents (which always remained below the solubility limit). Much less water wa...

The possibility and usefulness of sinple liquid models is reported. The importance of van der Waals forces for liquid water and aqueous solutions is discussed and compared with the solubility of water in nonpolar solvents and as well as of nonpolar solvents in water. Both systems could be understood as equilibrium of few water monomers. The network of H-bonded water molecules seems to be less i...

OBJECTIVE The purification of commercially prepared triamcinolone acetonide is important in order to avoid the potential toxic side-effects of the solvent benzyl alcohol. We present a new technique for preparation of pure triamcinolone acetonide by dissolving the powder in sterile distilled water with no additional solvents. As the triamcinolone powder is relatively insoluble in water, we descr...