Volume 1 • Issue 7 • 1000e125 Biochem Anal Biochem ISSN:2161-1009 Biochem, an open access journal Electrophoresis is one of the research techniques, which has extensively been used in separation and identification of proteins/ enzymes. In majority of the biochemistry and molecular biology experiments, electrophoresis of proteins and enzymes is performed using polyacrylamide gel electrophoresis ...

Sodium dodecyl sulfate (SDS) was introduced as an esterase inhibitor in the determination of two extremely labile, ultrashort-acting beta-blockers-esmolol and flumolol--in whole blood. SDS was much more efficient than previously used agents in preventing hydrolysis. The assay comprises solvent extraction, perfluoroacylation, and capillary gas-chromatography with electron-capture detection. The ...

We study dilute solutions of poly(ethy1ene oxide) (PEO) and sodium dodecyl sulfate (SDS) mixture in water with small angle neutron scattering (SANS) experiments. The attachment of the surfactant molecules to the polymers in the form of micelles results in a repulsive interaction within the polymers, which in turn results in interesting behavior of the viscosity as a function of the SDS concentr...

Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of nuclear non-histone proteins extracted with 0.15 and 0.30 M NaCl from human normal lymphocytes or leukemic leukocytes indicated a lack of bands in the highermolecular-weight regions of the gels. However, the corre sponding fractions from phytohemagglutinin-stimulated lymphocytes and Burkitt lymphoma cells did contain highmolecul...

The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl sulfate (SDS)-polyacylamide gel electrophoresis. It has a high molecular mass ...

Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is paralle...

We report the first vibrational sum frequency generation (VSFG) spectroscopic study from particle surfaces of powdered solids using a modified SFG approach, diffuse reflection broad bandwidth sum frequency generation (DR-BBSFG). The DR-BBSFG spectrum of sodium dodecyl sulfate (SDS, C(12)H(25)SO(4)Na) powdered solids was obtained. Five peaks were resolved by calculated fits. Possible origins of ...

This paper deals with the effects of a surfactant additive on the formation of methane hydrate in water system with and without sodium dodecyl sulfate (SDS). The properties of sodium dodecyl sulfate are listed. The results manifested that the presence of SDS could not only accelerate the hydrate formation process, but also increase the partition coefficient of methane between hydrate and vapor ...

To improve the magnesia removal effect of collophane and increase P2O5 grade phosphorus concentrate, fatty acid collector mixed with sodium dodecyl sulfate (SDS) was used as compound in reverse flotation for system. Solution surface tension measurement, Zeta potential test infrared spectrum analysis were conducted to analyze intensified dolomite removal. The results showed that under conditions...

In this study, the effect of these variables on commercial silica NP retention was presented in a fabricated flow model considering only physical adsorption aspects retention. From our observations, it established that while concentration, rate and salt are key influencing agglomeration retention, temperature highly subdued. The salt-induced particularly severe at moderate salinity (?4 wt% NaCl...