We report herein the separation of enantiomers of glycerol derivatives on a cellulose-modified chiral column (Chiracel OD) in normal-phase mode. This method is suitable for monitoring the progress of the reaction and for determination of the enantiomeric purity of products. The lipase Novozyme 435-catalysed enantioselective hydrolysis of 1-O-benzyl-2,3di-O-octanoyl-rac-glycerol has been shown t...

Polar ether lipids extracted from 15 methanogenic bacteria, representative of seven genera, were screened by nuclear magnetic resonance and thin layer chromatography for the presence of hydroxyl groups on the C20-phytanyl moieties. Major amounts of hydroxydiether core lipid were confirmed for Methanosaeta concilii and discovered in two Methanosarcina species, Methanococcus voltae, and tentative...

1-O-Hexadecyl-2-O-methyl-3-O-(2'-amino-2'-deoxy-β-D-glucopyranosyl)-sn-glycerol (1) was previously reported to show potent in vitro antitumor activity on a range of cancer cell lines derived from breast, pancreas and prostate cancer. This compound was not toxic to mice and was inactive against breast tumor xenografts in mice. This inactivity was attributed to hydrolysis of the glycosidic linkag...

Elevations in the mass of ether-linked diglycerides (i.e. 1-O-alk-1'-enyl-2-acyl-sn-glycerol (AAG) and 1-O-alkyl-2-acyl-sn-glycerol (Alkyl AG)) during cellular activation are prolonged in comparison to their 1,2-diacyl-sn-glycerol (DAG) counterparts. Since the metabolic removal of DAG is determined, in large part, by the rate of its phosphorylation by diglyceride kinase, we quantified differenc...

This paper deals with the results of quantum chemical modeling monoacyl-sn-glycerol 2D cluster formation at air/water interface using a semi-empirical PM3 method. The impact 2 or 3 positions acyl substituent on thermodynamics monolayer is assessed for surfactants an CnH2n+1COO chain length n = 6–17 carbon atoms. calculation shows significant change in spontaneous clusterization threshold isomer...

In the present work, we studied catalytic performance (activity and selectivity) of Pt supported on carbon systems modified with tin in glycerol oxidation H2O2 as oxidising agent at atmospheric pressure, 60 °C, base-free conditions. The conversion obtained monometallic catalyst was 37% remained unchanged addition Sn a ratio Sn/Pt = 0.4. two bimetallic PtSn catalysts were able to oxidise commerc...

Four Castanea sativa Miller cultivars (Aveleira, Boaventura, Judia and Longal) belonging to the Protected Designation of Origin "Castanha da Terra Fria", from the Northeast of Portugal, were selected in 2006, 2007 and 2008. Their nutritional, fatty acids, triacylglycerols and tocopherols profiles were evaluated. Water was the major component, followed by carbohydrates, protein and fat, with ene...

This review deals with the in vitro biosynthesis of the characteristics of polar lipids in archaea along with preceding in vivo studies. Isoprenoid chains are synthesized through the classical mevalonate pathway, as in eucarya, with minor modifications in some archaeal species. Most enzymes involved in the pathway have been identified enzymatically and/or genomically. Three of the relevant enzy...

The membrane-bound sn-glycerol-3-phosphate acyltransferase of Escherichia coli was purified to near homogeneity from Triton X-100 extracts of membranes from strain vL3/pvL1 (Lightner, V. A., Larson, T. J., Tailleur, P., Kantor, G. D., Raetz, C. R. H., Bell, R. M., and Modrich, P. (1980) d Biol. Chem. 255, 9413-9420). This strain contains a hybrid plasmid bearing the pZsB gene, a structural gene...