The lipids in the uppermost layer of the skin, the stratum corneum (SC), play an important role in the barrier function. The main lipid classes in stratum corneum are ceramides, cholesterol, and free fatty acids. In previous publications, a lipid model was presented, referred to as the stratum corneum substitute (SCS), that closely mimics the SC lipid organization and SC barrier function. In th...

Reactive oxygen species can be generated by daily exposure of the skin to ultraviolet light and may cause some subchronic and chronic skin disorders. The aim of this study was to investigate a possible preventive role of alpha-tocopherol acetate (ATA) on ultraviolet B (UVB) induced peroxidation by assessing lipid peroxide (LPO) levels and activity of reactive oxygen scavenging enzymes including...

the water content of the epidermis is a main factor in maintaining skin smoothness and elasticity and preventing skin dryness. occlusive products can greatly affect skin hydration by forming a barrier on the skin following the topical administration of oil-based formulations. these products repair the skin barrier by restoring the skin lipids as well. solid lipid nanoparticles (slns) have recen...

The skin is the largest organ of the body and has a very complex and heterogeneous structure. The innermost layer is the subcutaneous fat layer. Next is the dermis, which contains fibroblasts that produce collagen and elastic fibers. Localized within the dermis are specialized organelles including sebaceous glands, eccrine glands (sweat glands), and hair follicles. The nerves and blood vessels ...

A canine skin equivalent model has been validated for the assessment of a topical formulation effects. Skin equivalents were developed from freshly isolated cutaneous canine fibroblasts and keratinocytes, after enzymatic digestion of skin samples (n = 8) from different breeds. Fibroblasts were embedded into a collagen type I matrix, and keratinocytes were seeded onto its surface at air-liquid i...

The stratum corneum serves as a critical barrier between the host and its external environment. Rich in ceramides, is one of largest sphingolipid reservoirs body. Our recent work using bioorthogonal labeling strategy traced sphingolipids from gut commensal Bacteroides thetaiotaomicron (BT) to liver mice fed with fat-free/high-sucrose diet demonstrated direct transfer labeled BT-sphingolipid liv...

The skin is the largest organ of the body, whose main function is to protect the body against the loss of physiologically important components as well as harmful environmental insults. From the inside to the outside, the skin comprises three major structural layers: the hypodermis, the dermis and the epidermis. The epidermis contains four different sublayers, the stratum corneum (SC), stratum g...

BACKGROUND Cellulite is seen in more than 85% of postpubertal women. Recent studies show that bipolar radiofrequency and low-level laser devices can produce mild skin tightening of cellulite. OBJECTIVE The primary objective was to determine if a novel unipolar, more deeply penetrating, radiofrequency device can promote better skin tightening with fewer treatments than is seen with previously ...

Objective: This work compares the occlusive effect and the penetration enhancement ability of solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC), through in vitro skin. Methods: SLN and NLC were prepared by high shear homogenization and characterized by size, polydispersity index, zeta potential, morphology and physical stability. Occlusive effect was assessed by an in vitr...

Pathogenic memory T cells are implicated in the local of chronic skin diseases. The factors underlying their persistence and function human resolved epidermis not understood. Lipid composition transcriptional programs lipid metabolism known to be altered active non-lesional psoriasis, faculty resident uptake fatty acids is essential survival. Here, we wished correlate functionality epidermal ps...