Expressed sequence tags simple sequence repeats (EST-SSRs) are important sources for investigation of genetic diversity and molecular marker development. Similar to genomic SSRs, the EST-SSRs are useful markers for many applications in genetics and plant breeding such as genetic diversity analysis, molecular mapping and cross-transferability across related species and genera. In spite of low po...

DNA methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. To assess DNA methylation pattern in different developmental stages of Aeluropus littoralis, methylation sensitive amplified polymorphism (MSAP) was used. Methylation and demethylation status at the CCGG recognition site were...

to evaluate the genetic diversity of native populations of crown imperial (fritillaria imperialis l.) in zagross region, issr molecular along with 32 morphological traits were employed. a number of 160 samples corresponding to eight zagross populations distributed in six provinces were collected and assessed. using six primers, 57 bands were totally produced from which 49 were polymorphic. mean...

Little is known about the genetics or genomics of Panax ginseng. In this study, we developed 70 expressed sequence tag-derived polymorphic simple sequence repeat markers by trials of 140 primer pairs. All of the 70 markers showed reproducible polymorphism among four Panax speciesand 19 of them were polymorphic in six P. ginseng cultivars. These markers segregated 1:2:1 manner of Mendelian inher...

  Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic relationships between two seasonal immigrant forms (namely fall-run and spring-run) of Caspian trout Salmo trutta caspius. In this regard, 62 fin tissue samples were collected from southern parts of the Caspian Sea basin and examined with 16 oligodecamer primers to asses their genetic diversity as well as probable s...

Randomly amplified polymorphic DNA (RAPD) markers were used to study the genetic diversity and discriminate among 17 unknown genotypes (considered potentially salt tolerant) and 16 known olive cultivars. Fifteen decamer primers which produced 38 reproducible polymorphic bands in the genotypes were selected for analysis. The RAPD markers resulted in 93 distinct banding patterns. Based on either ...

The olive tree is an iconic tree of the Mediterranean, and is used extensively to produce high-quality olive oil. Although the China olive industry has just begun to be valued, there were also existed mislabeling and synonyms in introduced cultivars. The aim of this study was to analyze genetic similarities among olive cultivars in China using SSR and ISSR techniques. Thirty-two samples were co...

expressed sequence tags simple sequence repeats (est-ssrs) are important sources for investigation ofgenetic diversity and molecular marker development. similar to genomic ssrs, the est-ssrs are usefulmarkers for many applications in genetics and plant breeding such as genetic diversity analysis, molecularmapping and cross-transferability across related species and genera. in spite of low polym...

to investigate the genetic diversity of 21 rapeseeds (brassica napus l.), 20 semi-random isj and ssr primers were used. primers produced a total number of 158 (81.5%) polymorphic bands (51 ssr and 107 isj bands). average numbers of polymorphic bands for isj, ssr and combined of them were 10.7, 5.1 and 7.9, respectively. pic values ranged from 0.0150 for specific primer o110d08 to 0.3420 for sem...

Genetic variability among the genotypes of any species could be utilized for its improvement. PCR-based Random Amplified Polymorphic DNA (RAPD) technique was used to determine the genetic diversity and relationship among 10 cucumber varieties and genotypes. Five decamer primers were used to amplify genomic DNA and the primers yielded a total of 54 bands of which 36 bands were polymorphic and 18...