نتایج جستجو برای: sitedirected mutagenesis
تعداد نتایج: 27679 فیلتر نتایج به سال:
The OB-fold is a diverse structure superfamily based on a beta-barrel motif that is often supplemented with additional non-conserved secondary structures. Previous deletion mutagenesis and NMR hydrogen exchange studies of three OB-fold proteins showed that the structural stabilities of sites within the conserved beta-barrels were larger than sites in non-conserved segments. In this work we exam...
macrophomina phaseolina is one of the major yield limiting factors of melons in tropical and subtropical regions. for eco-friendly and effective management of the disease, 24 gamma induced mutants from trichoderma harzianum were evaluated against three isolates of the pathogen representing three geographically different regions viz. khorasan (isolate 1), garmsar (isolate 2) and khuzestan (isola...
A major challenge in the post-genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out expression of a gene with the hope of seeing a phenotype that is suggestive of its function. Insertional mutagenesis is a useful tool for this type of study, but it is limited by gene redundancy, lethal...
1Department of Genetics, Cell Biology and Development and Department of Pediatrics, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 2Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI; 3Biostatistics and Bioinformatics, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 4Department of Hemat...
High-throughput technologies of functional genomics such as T-DNA insertional mutagenesis and microarray expression profiling have been employed to identify genes related to pathogenicity in Magnaporthe oryzae. However, validation of the functions of individual genes identified by these high-throughput approaches is laborious. In this study, we compared two published lists of genes putatively r...
Abstract Teratological spores and pollen are widespread in sediments that record the Permian-Triassic mass extinction. The malformations thought to be result of extreme environmental conditions at time, but mutagenic agents precise timing events remain unclear. We examined abundance teratological sporomorphs metal concentrations a tropical peatland succession southwestern China. find significan...
Post-translational modifications of proteins are ubiquitous in living organisms, as they enable an accurate control the interactions these macromolecules. For mechanistic studies, it would be highly advantageous to able produce vitro post-translationally modified with site-specificity. Here, we demonstrate one facile way achieve this goal through use post-translational chemical mutagenesis. We ...
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