Redox-responsive NPs, delivering DTX in combination with TUBB3 siRNA, increased activity lung cancer (LC) cells. After local administration LC mice models, NPs were retained into the lungs thus exerting high siRNA silencing efficacy.

Small interfering RNAs (siRNA) have enabled novel, specific, and efficient treatment of diseases, including cancer. To obtain sufficient well-controlled intracellular delivery, a drug delivery system is essential. Herein for siRNA, composed cationic biodegradable nanogels, presented. P(DEAEMA-co-HEMA-g-PEGMA) nanogels with varying ratios 2-diethyl aminoethyl methacrylate (DEAEMA) 2-Hydroxyethyl...

background: rna interference-based gene silencing has recently been applied as an efficient tool for functional gene analysis. rorc2 is the key transcription factor orchestrating th17 cells differentiation, the cells that are known as the pathogenic elements in various autoimmune diseases. the aim of this study was to design efficient sirnas specific for rorc2 and to evaluate different criteria...

In this study, we investigated the ability of curcumin alone or in combination with GLUT1 siRNA to radiosensitize laryngeal carcinoma (LC) through induction autophagy. Protein levels tumour tissues and LC cells were measured by immunohistochemistry Western blotting. vitro, cell proliferation, colony formation assays, death autophagy detected. A nude mouse xenograft model was established injecti...

In this paper, we describe denaturing and non-denaturing ion-pair reversed-phase liquid chromatography (RPLC) methods for analyzing small interfering ribonucleic acid (siRNA). Drug formulations consisting of one or two siRNA duplexes were analyzed in conditions at a low column temperature to separate intact from single-stranded oligonucleotide contaminants quantify them. method, used an elevate...