We demonstrate the effects of protein orientation and trehalose on a quantitative analysis of surface-immobilized proteins by using time-of-flight secondary ion mass spectrometry (TOF-SIMS). As our model protein, streptavidin (SA) was quantitatively immobilized on a solid surface at different configurations by random or oriented immobilization and subsequently treated with trehalose. The result...

The rapid adoption of social media by billions of people from all over the world has unleashed unprecedented opportunities for marketers and cognitive scientists to better understand why some message become popular while other die quickly. We designed a novel technique for automatically learning to differentiate popular tweets from unpopular ones and to predict how popular a given tweet will be...

S OF REPORTS FROM BSA VACATION

ToF-SIMS has been used in the analysis of latent fingerprints (dactyloscopy) with a view to use in forensic and biomedical applications. Szynkowska et al have demonstrated that SIMS can be employed to produce images of whole fingerprints on inorganic surfaces such as steel, brass and glass (1). We demonstrate the detection of a range of exogenous materials in fingerprints including industrial l...

Chemical imaging of single cells at the molecular level is important in capturing biological dynamics. Single cell correlative imaging is realized between super-resolution microscopy, namely, structured illumination microscopy (SIM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS) using a multimodal microreactor (i.e., System for Analysis at the Liquid Vacuum Interface, SALVI). SI...

Boyland, E. & Manson, D. (1951). J. chem. Soc. p. 1837. Boyland, E., Ramsay, G. S. & Sims, P. (1961). Biochem. J. 78, 376. Boyland, E. & Sims, P. (1958). Biochem. J. 68, 440. Boyland, E. & Sims, P. (1960). Biochem. J. 77, 175. Boyland, E. & Sims, P. (1961). Rep. Brit. Emp. Cancer Campgn, 89, 50. Boyland, E. & Sims, P. (1962a). Biochem. J. 84, 564. Boyland, E. & Sims, P. (1962b). Biochem. J. 84,...

FIB-based instruments play an ever more important role in materials science and also in life science. While such FIB-based instrumentation is an ideal tool for high resolution imaging (2D and 3D imaging) and nanofabrication (nanomaching, nanodeposition, specimen preparation), its analysis capability is currently limited. By contrast, Secondary Ion Mass Spectrometry (SIMS) is an extremely powerf...