Although mammalian cell factories are now widely used in production of biomedically important proteins they are still relatively ineffective compared with those from prokaryotic organisms. In an attempt to improve the CHO cell line we have used a biotechological approach to investigate whether or not the choice of signal peptide may have a major impact on the synthesis/ secretion of a recombina...

Carbon nanotube (CNT) chemiresistors have emerged as miniaturized platforms for wearable volatile organic compound (VOC) sensors. As a promising biorecognition element (BRE), short peptide can functionalize CNT to be sensitive and selective target VOCs. However, unveiling the VOC-optimized peptide-CNT pair gas-phase sensing remains unclear. Here, novel multimodal molecular toolset designing, bu...

SecA signal peptide interaction is critical for initiating protein translocation in the bacterial Sec-dependent pathway. Here, we have utilized the recent nuclear magnetic resonance (NMR) and Förster resonance energy transfer studies that mapped the location of the SecA signal peptide-binding site to design and isolate signal peptide-binding-defective secA mutants. Biochemical characterization ...

The presence of signal peptide in the query sequence complicates the transmembrane (TM) topology prediction because the hydrophobic core of signal peptide is easily predicted as the putative first TM segment (Lao and Shimizu, 2001). In genome wide analyses, the likely signal peptide region is treated in several ways. It was either masked out from topological calculations (Jones, 1998) or omitte...

In previous investigations, we have examined the effect of OmpA signal peptide mutations on the secretion of the two heterologous proteins TEM beta-lactamase and nuclease A. During these studies, we observed that a given signal peptide mutation could affect differentially the processing of precursor OmpA-nuclease or precursor OmpA-lactamase. This observation led us to further investigate the in...

The pCloDF13-derived bacteriocin release protein (BRP) is synthesized as a prelipoprotein with a signal peptide which remains stable after processing. This signal peptide accumulates in the cytoplasmic membrane and is, together with the mature BRP, required for efficient release of cloacin DF13. We investigated the structural requirements for stability of the BRP signal peptide by constructing ...

CD4+ T cells transfected with the C-terminal 130 aa of human IL-16 are rendered resistant to HIV infection. Whether the constitutively expressed IL-16 acts intracellularly, extracellularly, or both is not clear. To address this question and to further study the processing of IL-16, new constructs containing either the C-terminal 130 aa or the C-terminal 100 aa (PDZ-like motif) were constructed ...