نتایج جستجو برای: shv بتالاکتاماز

تعداد نتایج: 1355  

Journal: :Journal of clinical microbiology 2002
Mariagrazia Perilli Emanuela Dell'Amico Bernardetta Segatore Maria Rosaria de Massis Ciro Bianchi Francesco Luzzaro Gian Maria Rossolini Antonio Toniolo Giuseppe Nicoletti Gianfranco Amicosante

Extended-spectrum beta-lactamases (ESBLs) are widespread in hospital settings worldwide. The present investigation was undertaken to assess the distribution and prevalence of ESBLs belonging to the TEM and SHV families in 448 ESBL-producing clinical isolates of Enterobacteriaceae collected from 10 different Italian hospitals. The natures of TEM and SHV determinants were identified by direct seq...

ژورنال: :مجله دانشگاه علوم پزشکی شهرکرد 0
عباس دوستی abbas doosti shahrekord azad universityدانشگاه آزاد شهرکرد معصومه داریوشی masoumeh daryousi islamic azad university of jahromدانشگاه آزاد جهرم رضوان برزا rezvan barza islamic azad university of jahromدانشگاه آزاد جهرم مریم پسند maryam pasand islamic azad university of shahrekordدانشگاه آزاد شهرکرد

زمینه و هدف: عفونت دستگاه ادراری از شایع ترین عفونت ها در زنان و کودکان است که در این میان باکتری اشریشیاکلی عامل اصلی عفونت بشمار می رود. مقاومت علیه آنتی بیوتیک های مورد استفاده، مشکل مهمی در پروسه درمانی می باشد. این مطالعه به منظور تعیین مقاومت آنتی بیوتیکی و بررسی شیوع ژنهای مقاومت نسبت به آنتی بیوتیک ها می باشد. روش بررسی: مطالعه توصیفی-مقطعی از تعداد 95 بیمار زن و کودک مبتلا به عفونت ادر...

چکیده    مقاومت ضدمیکروبی ایجاد شده به­واسطه تولید آنزیم­های بتالاکتاماز وسیع­الطیف (ESBLs) در اشریشیا کلی یک تهدید اساسی در کلی­باسیلوزیس ماکیان محسوب می­شود. مطالعه حاضر به شناسایی ژن­های blaTEM، blaSHVو blaOXAدر جدایه­های اشریشیا کلی به­دست آمده از کلی­باسیلوزیس طیور با استفاده از روش MPCR می­پردازد. در مجموع، 60 جدایه اشریشیا کلیاز طیور استان کرمان به­دست آمد. DNA سلولی با استفاده از کیت D...

Journal: :Antimicrobial agents and chemotherapy 2001
C C Randegger H Hächler

Extended-spectrum beta-lactamases (ESBLs), e.g., ESBLs of the TEM or SHV type, compromise the efficacies of expanded-spectrum cephalosporins. An SHV non-ESBL that hydrolyzes only narrow-spectrum cephalosporins can be converted into an SHV ESBL through substitutions at three amino acid positions, 179, 238, or 238--240. In order to improve detection of SHV ESBLs, a novel method, based on real-tim...

Journal: :Journal of clinical microbiology 2006
Nashwan Al Naiemi Kim Schipper Birgitta Duim Aldert Bart

Nucleotide sequencing is the standard molecular method for determination of the beta-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of bla(SHV) genes, as illustrated by three strains of three different species that each contained two different bla(SHV) alleles, SHV-2 and SHV-12.

Journal: :Antimicrobial agents and chemotherapy 2008
Véronique Dubois Laurent Poirel François Demarthe Corinne Arpin Laure Coulange Luciene A R Minarini Marie-Christine Bezian Patrice Nordmann Claudine Quentin

A clinical strain of Klebsiella pneumoniae was found to possess the chromosomal gene bla(SHV-56), encoding a new inhibitor-resistant beta-lactamase with a pI of 7.6. SHV-56 is derived from SHV-11 by the single substitution K234R. This mutation therefore evidences a new critical site for inhibitor resistance among SHV enzymes.

Journal: :The Indian journal of medical research 2007
Prabha Lal Arti Kapil Bimal K Das Seema Sood

BACKGROUND & OBJECTIVE Extended-spectrum beta-lactamases (ESBLs) are rapidly evolving group of beta-lactamase enzymes produced by the Gram negative bacteria. These enzymes have been derived from TEM and SHV genes by mutations and have been well described in Klebsiella pneumoniae. Information on molecular types of ESBL positive Klebsiella sp. is lacking from India. We therefore undertook this st...

Journal: :Antimicrobial agents and chemotherapy 2000
C C Randegger A Keller M Irla A Wada H Hächler

SHV extended-spectrum beta-lactamases (ESBLs) arise through single amino acid substitutions in the parental enzyme, SHV-1. In order to evaluate the effect of genetic dissimilarities around the structural gene on MICs, we had previously devised an isogenic system of strains. Here, we present an extended version of the system that now allows assessment of all major types of SHV beta-lactamases as...

2014
Nahed Ben Achour Omrane Belhadj Moreno Galleni Mohamed Ben Moussa Paola Sandra Mercuri

Klebsiella pneumoniae ML2011, a multiresistant isolate, was isolated from the Military Hospital of Tunis (Tunisia). The determination of the minimal inhibitory concentrations exhibited by K. pneumoniae ML2011 was performed by Etest. The crude extract of the isolates contains four different β -lactamases with pI 5.5, 7.3, 7.6, and 8.6. Only the β -lactamases with pI 7.3 and pI 8.6 were transferr...

Journal: :Microbial drug resistance 2011
Fernanda M Tollentino Milena Polotto Mauricio L Nogueira Nilton Lincopan Patrícia Neves Elsa M Mamizuka Gisele A Remeli Margarete T G De Almeida Fernando G Rúbio Mara C L Nogueira

The aim of this study was to investigate the presence and prevalence of bla(TEM), bla(SHV), and bla(CTX-M) and bla(GES)-like genes, responsible for extended spectrum beta-lactamases (ESBLs) production in clinical isolates of Klebsiella pneumoniae collected from a Brazilian tertiary care hospital. Sixty-five ESBL producing K. pneumoniae isolates, collected between 2005 and 2007, were screened by...

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