Flowering control is one of the several strategies for gene containment of transgenic plants. TERMINAL FLOWER 1 (TFL1) is known to be involved in the transcriptional repression of genes for inflorescence development. Two TFL1 transcripts with different 3' UTR were cloned from black cherry (Prunus serotina Ehrh.) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplificat...

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials an...

In vitro propagation of Tilia platyphyllos Scop. has been achieved by axillary shoot proliferation and somatic embryogenesis. The influence of tree age, explant source, genotype, and phytohormones on micropropagation of juvenile and mature trees of Tilia platyphyllos has been investigated. Nodal segments and shoot tips were used as initial explants for axillary shoot proliferation. Low concentr...

In the present study best suitable explant for encapsulation and effect of nutrient composition of alginate matrix on regrowth performance of encapsulated explants of endangered C. spiralis and C. pusilla were studied. Among all the explants tested, good sprouting frequency was observed with shoot tips in both the species. Where as, multiple shoot induction with maximum shoot length was noticed...

A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explant...

Abstract The current study was conducted with aim to find an efficient simple protocol for propagation of stevia plant ( Stevia rebaudiana ) In Vitro . Shoot tips, leaves and stem nodes explants were sterilized different concentrations NaOCl (0.0, 2.0, 4.0 6% v/v) then cultured on MS medium supplemented 30 g.l -1 sucrose. Results revealed that, no contamination recorded when using % 6.0 (100% 8...