BACKGROUND Homology-dependent selective degradation of RNA, or post-transcriptional gene silencing (PTGS), is involved in several biological phenomena, including adaptative defense mechanisms against plant viruses. Small interfering RNAs mediate the selective degradation of target RNA by guiding a multicomponent RNAse. Expression of self-complementary hairpin RNAs within two complementary regio...

RNA-Seq has proven to be a very powerful tool in the analysis of the Plum pox virus (PPV, sharka disease)/Prunus interaction. This technique is an important complementary tool to other means of studying genomics. In this work an analysis of gene expression of resistance/susceptibility to PPV in apricot is performed. RNA-Seq has been applied to analyse the gene expression changes induced by PPV ...

In order to fill in a decade-long information gap regarding the biological, serological and molecular data for plum tree viruses Bosnia Herzegovina, three-phase study combining symptom evaluation, assays with high-throughput sequencing (HTS) technology was conducted. The most frequently observed symptoms were discolorations form of ring patterns, bands irregular shapes, as well vein banding. Sh...

The Tilia genus (linden, lime) comprises about 30 deciduous tree species native to North America, Europe, and Asia. They are often used along roads in parks because of their aesthetic value urban landscapes. During surveys 2017–2018 Istanbul, Turkey, typical viral disease symptoms such as foliar mosaics, mottling chlorotic ringspots, deformations were observed on spp. (Figs. 1-3). Forty-six lea...

Research to date indicates that post-transcriptional gene silencing (PTGS) is an effective strategy for the development of stable high-level resistance to Plum pox virus in plum. Field tests in Poland, Romania, and Spain show that after 5–6 years of natural aphid vectored inoculation, trees of the PTGS clone C5 remain virus-free. The effectiveness of transgene-based PTGS for imparting potyvirus...