Drought and other types of abiotic stresses negatively affect plant growth and crop yields. The abscisic acid-, stress-, and ripening-induced (ASR) proteins play important roles in the protection of plants against abiotic stress. However, the regulatory pathway of the gene encoding this protein remains to be elucidated. In this study, the foxtail millet (Setaria italica) ASR gene, SiASR4, was c...

Background: CRISPR activation (CRISPRa) uses non-functional Cas9 endonuclease (dCas9) but retains the genome targeting ability through its single guide RNAs (sgRNAs). CRISPRa is widely used as a gene system exploiting in recruiting various transcriptional domains (TADs) for enhancing expression of target gene(s). Drought tolerant and resource-efficient crops like millets have potential mitigati...

Prolamin, the alcohol-soluble seed storage protein is major in many cereals. Foxtail millet (Setaria italica) contains prolamin (Setarin) as protein, which accounts > 50% of total protein. To in-silico characterize proteins foxtail millet, sequence coding for 14.3 kDa, 20.4 22 22.4 kDa and 26 were retrieved from NCBI database. Protein physio-chemical characterization was done through Protparam ...