AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

AIM The objective of this research is to report parameters for breeding soundness evaluation, semen extension, and cryopreservation in Rusa timorensis. MATERIALS AND METHODS Seven healthy stags were chosen for semen collection using an electroejaculator. The collections were performed twice in a breeding season between February and June 2016. Samples were collected between 2 and 3 weeks inter...

The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in extender on post-thaw quality of buffalo spermatozoa. Semen was collected from three buffalo bulls with artificial vagina (42°C) for three weeks (replicates). Qualifying semen ejaculate from each bull was split into five aliquots and diluted in tris-citric egg yolk extender containing ...

Background: Nowadays, cryoprotectant-free vitrification of human sperm in large or small volumes facilitated storage and use of human sperm for long time. However, this procedure was not successful in farm animals. The main finding in the studies is higher sensitivity of these kinds of sperm to sucrose osmolarities. Preliminary studies showed a reversible effect of sucrose on goat epididymal sp...

This study was carried out to identify the suitable buffer for cryopreservation of buffalo semen. Semen was collected with artificial vagina (42 degrees C) from four buffalo bulls. Split pooled ejaculates (n=5), possessing more than 60% visual sperm motility, were extended at 37 degrees C either in tri-sodium citrate (CITRATE), Tris-citric acid (TCA), Tris-Tes (TEST) or Tris-Hepes (HEPEST). Sem...

Background: Trehalose, a non-permeable disaccharide, has a protective role related both to osmotic effect and specific interactions with membrane phospholipids, rendering hypertonic media, causing cellular osmotic dehydration before freezing, and then decreasing the amount of cell injury by ice crystallization. Within this study, an attempt was made to evaluation of two levels of 50 and 100 mM ...

the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from ...