AIM The objective of this study was to evaluate the effects of different concentrations of soybean lecithin (SL) and virgin coconut oil (VCO) in Tris-based extender on chilled and frozen-thawed bull semen quality parameters. MATERIALS AND METHODS A total of 24 ejaculates were collected from four bulls via an electroejaculator. Semen samples were diluted with 2% VCO in Tris-based extender whic...

the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from ...

The aims were to evaluate the suitability of a non-commercial TRIS-lecithin (LC) extender and the effect of different concentrations of catalase (CAT) on motility, capacitation status (Chlortetracycline-assay) and zona pellucida (ZP) binding capacity of canine spermatozoa stored at +5 degrees C for 4 days. The sperm-rich fractions of the ejaculates of four stud dogs were divided into four aliqu...

Camels represent part of the Arab heritage. The interest in developing assisted reproductive technologies and cryobanking for the conservation of animal genetic resources has recently increased. However, semen collections in camelids present many problems as sitting position during copulation, slow ejaculation and difficult animal handling. In these cases epididymal sperm from slaughtered or re...

The aim of this study was investigation of various levels of vitamin E in Tris extender on semen characteristics of Ghezel rams in pre and post freezing conditions. Semen was collected from six healthy and mature rams with average 55±5 Kg body weight by electro ejaculator. High quality samples were mixed and diluted in Tris extender after apply different concentration of vitamin E. Treatments w...

Background: Frozen-thawed rooster semen is not reliable for use in artificial insemination in commercial stocks. Low density lipoprotein (LDL) has been assessed for effectiveness as a cryoprotectant in the extender to improve the quality of frozen-thawed rooster semen. Although LDL has been evaluated in a few studies in other species for semen cryopreservation, to date, no study has been conduc...

The present work was designed to study the effect of different concentrations of bovine serum albumin (BSA) in extender on post-thaw quality of buffalo spermatozoa. Semen was collected from three buffalo bulls with artificial vagina (42°C) for three weeks (replicates). Qualifying semen ejaculate from each bull was split into five aliquots and diluted in tris-citric egg yolk extender containing ...

Background: This study was carried out to assess the effects of MnTBAP, a cell permeable antioxidant, on motility, membrane integrity, capacitation status and in vitro fertilization ability of frozen-thawed ram semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from five rams, mixed and divided into five equal fractions, and diluted (1:20 v/v) with commer...

The aim of this study was to evaluate the effects of the addition of antioxidants (vitamin E, β- mercaptoethanol and their combination) to semen extender.  Progressive motility, viability and pH of fresh ram semen were examined. Ejaculate samples were collected with artificial vagina from three Ghezel-Merinose rams (3 years old) during non- breeding reproduction season. Semen was diluted with a...