OBJECTIVE To review the literature and answer questions about semen quality in young cancer patients before and after therapy and the importance of sperm cryopreservation. METHODS All aspects of sperm cryopreservation and effects of therapies on semen quality were examined on the basis of MedLine database searches. RESULTS Chemotherapy, radiation, or their combination results in a significa...

The present study was to improve the success for the cryopreservation of stallion spermatozoa, especially as the costs of using low post-thaw motility or sub-optimal fertility deters owners and breeders from using frozen semen. The aim was to develop a freezing extender/process that supports cell survivability throughout the freezing. Eleven stallions were collected and frozen in INRA 96 with t...

BACKGROUND In France, assisted reproductive technologies involving a hepatitis C virus (HCV)-infected man requires the cryopreservation of potentially infected semen (in order to establish the presence of HCV), hence the need for a safe and secure storage system. We evaluated the safety of high-security straws for the conservation of semen containing HCV RNA under routine conditions. METHODS ...

Cryopreservation of sperm has many applications in agriculture, biotechnology, and clinical medicine. The spermatozoon is a very specialized cell that loses the ability of biosynthesis, repair, growth and cell division during the final phase of spermatogenesis. Cryopreservation of sperm generally requires a reduction or arrest of the metabolism of cells, thereby prolonging their life. Semen sam...

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signed to alert university-based administrators of the value of poultry genetic resources. The committees’ present administrative goals include development of priority guidelines for repository deposits of semen at the Ft. Collins NAGP facility, development of poultry-specific parameters for the GRIN database, establishment and distribution of useful guidelines/advice for handling and cryoprese...

Successful cryopreservation for human spermatozoa markedly influences the reproductive outcomes of assisted reproductive technologies. But in spite of its usefulness, cryopreservation significantly decreases sperm quality. l-carnitine has been found to improve the quality of spermatozoa in selected cases with male infertility. Here, we examined the efficacy of l-carnitine in improving sperm mot...

Introduction Although dog was the first species to be used for Artificial Insemination (AI) and become pregnant by Spallanzani in 1780, very little work has been done on preservation of dog semen to exploit the full genetic potential of pedigree males. Frozen semen is now inseminated routinely in cattle, sheep and goats and much research has been carried out with regard to the optimum condition...

OBJECTIVE To evaluate the effect of cryopreservation on sperm motility and viability and to assess sperm DNA fragmentation and oxidation in men undergoing infertility investigation before and after cryopreservation in liquid nitrogen. DESIGN Analysis of cryopreservation effects on sperm DNA integrity. SETTING Laboratory of Histology-Embryology of medicine faculty, Sfax, Tunisia. PATIENT(S...

Heparin binding proteins (HBPs) are produced by accessory glands. These are secreted into the seminal fluid, bind to the spermatozoa at the time of ejaculation, favour capacitation, acrosome reaction, and alter the immune system response toward the sperm. The present study was conducted with an objective to assess the effect of purified seminal plasma-HBPs (SP-HBPs) on cross bred cattle bull sp...