Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveolin-1 and sterol carrier protein-2 (SCP-2), a protein that binds and transfers b...

With just one eighth the size of the major capsid protein (MCP), the smallest capsid protein (SCP) of human tumor herpesviruses--Kaposi's sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV)--is vital to capsid assembly, yet its mechanism of action is unknown. Here, by cryoEM of KSHV at 6-Å resolution, we show that SCP forms a crown on each hexon and uses a kinked helix to cross-l...

Host factors that enable dengue virus (DENV) to propagate in the mosquito host cells are unclear. It is known that cellular cholesterol plays an important role in the life cycle of DENV in human host cells but unknown if the lipid requirements differ for mosquito versus mammalian. In mosquito Aedes aegypti, sterol carrier protein 2 (SCP-2) is critical for cellular cholesterol homeostasis. In th...

The yeast Saccharomyces cerevisiae is a potential candidate for converting waste resources into food-grade single cell protein (SCP). Food fermentation broth rich in lactic acid was tested as an alternative substrate source S. this study. An electrodialysis (ED) system developed to recover lactate, acetate and ammonium ions (La-, Ac- NH4+) from the broth. First, we evaluated resilience under va...

Single cell protein (SCP) is a good source of essential amino acids. This study aimed to utilize some date by-products such as fruit flesh produce single protein.  Fruit three unknown dates and varieties were used by Saccharomyces cerevisiae ATCC64712 under different condition (sugar concentrations in the extract media, temperatures pH values). The results showed that optimum production biomass...

Introduction Interest in the nutritional significance of dietary nucleic acids and their constituent purine and pyrimidine bases dates back to 1937 when Frost & Elvehjem ( I 93 7) demonstrated the beneficial properties of exogenous nucleotides given to rats fed on low-niacin diets. The early work formed part of the search for unidentified growth factors (UGF) in various dietary ingredients incl...

Sea cucumber is rich in protein that can be used to prepare a potential derived bioactive peptide for antioxidant and protective effect against UV-B induced skin cell damage. This study aimed optimize preparation of sea hydrolysate with both activities using three commercial enzymes response surface methodology (RSM) face-centered central composite design (face- centered CCD). Hydrolysis time c...

Sindbis virus core protein (SCP) has been isolated from virus and crystallized. The X-ray crystallographic structure showed that the amino-terminal 113 residues appeared to be either disordered or truncated during crystallization and that the carboxy-terminal residues 114 to 264 had a chymotrypsin-like structure. The carboxy-terminal residues 106 to 264 and 106 to 266 of SCP have now been expre...