The effect of temperature shift on Polyporus umbellatus sclerotial development was investigated. Micromorphology of the sclerotia was observed by using scanning electron microscopy (SEM). The cytochemical localization of H2O2 expressed as CeCl3 deposition at the subcellular level was observed by using transmission electron microscopy (TEM). Nox gene expression in sclerotia and mycelia was detec...

G-protein alpha subunits are involved in transmission of signals for development, pathogenicity, and secondary metabolism in plant pathogenic and saprophytic fungi. We cloned two G-protein alpha subunit genes, tgaA and tgaB, from the biocontrol fungus Trichoderma virens. tgaA belongs to the fungal Galphai class, while tgaB belongs to the class defined by gna-2 of Neurospora crassa. We compared ...

A development-specific protein (SSP) makes up about 35 to 40% of the total protein in sclerotia of the fungus Sclerotinia sclerotiorum. The protein consists of three charge isomers, with one isomer making up 80 to 90% of the total. In vitro translation of poly(A)+ RNA isolated from cells in early stages of sclerotia formation revealed that 44% of the amino acids incorporated was into SSP. In vi...

Alderman, S. C., Walenta, D., Hamm, P. B., Martin, R. C., Dung, J., and Kosman, E. 2015. Afternoon ascospore release in Claviceps purpurea optimizes perennial ryegrass infection. Plant Dis. 99:1410-1415. In Kentucky bluegrass (Poa pratensis),Claviceps purpurea, the causal agent of ergot, typically releases ascospores during the early-morning hours, between about midnight and 10:00 A.M., corresp...

charcoal rot disease, caused by macrophomina phaseolina has become a restrictive agent for annual crops in iran. the disease incidence reaches up to 90 percent in some infested areas. high air temperature and drought stress associated with this disease, are the main factors for increasing the disease incidence and severity. in order to isolate the pathogenic agents, pieces of infected root and ...

Trichoderma virens is a mycoparasitic fungus used in biocontrol of soilborne plant pathogens. It inhibits or kills plant-pathogenic fungi through production of antifungal antibiotics and parasitism of hyphae and sclerotia. Conidiation, or the production of asexual spores, an inducible process triggered by light or nutrient stress, is an important trait in survival and also development of formul...

Three kinds of sclerotia of Typhula spp. from Ardabil, East Azerbaijan and Qazvin Provinces in the northwest of Iran were collected. Morphology and ITS sequences of rDNA suggested that the collected fungi belonged to Typhula incarnata Lasch, Typhula phacorrhiza (Reichardt) Fr. and Typhula sp. These three taxa are new records in the Middle East and the result of this research suggests that Iran ...

The toxicity of sorghum ergot (Claviceps africana) was assessed in young pigs over 28 days. Fortyeight pigs of both sexes and 2 breeds (Large White and Duroc) were allocated across 6 grower diets, balanced for fibre and predicted digestible energy, and containing 0, 0.3, 0.6, 1.3, 2.5, or 5% ergot sclerotia [the 5% sclerotia diet contained 70 mg alkaloids/kg (>90% dihydroergosine)]. Blood sampl...

Phlebopus portentosus is a favorite wild, edible mushroom in the tropical region of China and northern Thailand. P. only bolete Boletales order that has been commercially cultivated. Sclerotia produced by are often found its natural habitats cultivated media. These sclerotia play key role life cycle. However, regularity growth characteristics sclerotium unknown. In this paper, whole process bir...