The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There is a lack of molecular information on the pineapple's response to cold stress. In this study, high-throughput transcriptome sequencing and gene expression analysis were performed on plantlets of a cold-tolerant genotype of the pineapple cultivar 'Shenwan'...

A new species, Cheilocostus borneensis, is described. Specimens were collected in Sarawak in 1987 and Kalimantan in 2000, but only intensified surveys of gingers in Sarawak in 2002-2004 provided sufficient collections to recognize the new species, which is here described and illustrated. It is closely related to the widespread C. globosus from which it differs by the chocolate-brown sheaths, ab...

Fecal progesterone assays were conducted in 3 captive female sun bears (Helarctos malayanus) segregated from males to characterize the species-specific reproductive pattern in their original distribution area in Sarawak, Malaysia. Peaks of fecal progesterone concentrations were observed once annually, and lactation was observed after increasing progesterone concentrations in all females without...

Noé López-Amador1, Cirilo Nolasco-Hipolito2, Macario de J. Rojas-Jimeno3, Octavio CarvajalZarrabal3* 1Institute of Forensic Medicine, University of Veracruz, SS Juan Pablo II s/n, 94294 Boca del Río, Ver., México 2Department of Chemical Engineering and Energy Sustainability of the Faculty of Engineering, University Malaysia Sarawak (UNIMAS), Kota Samarahan, Sarawak, Malaysia 3Biochemical and Nu...

Pineapple, Ananas comosus (L.) Merr., is a perennial, xerophytic monocot in the Bromeliaceae family (13). Planting materials used for commercial production are typically the crown from the top of the fruits (19). The planted crowns, referred to as the plant crop, will each produce a single pineapple fruit 18 to 20 months after planting (19). Following plant crop harvest, a ratoon crop develops ...

The cytochrome oxidase subunit I (COXI) gene sequences of three recent (20072008) clinical Plasmodium knowlesi isolates from Klang Valley, peninsular Malaysia, were determined and compared with those of older (1960’s) peninsular Malaysia, recent isolates from Sarawak (on Borneo Island), and an isolate from Thailand. Multiple alignment of the sequences showed that the three clinical isolates wer...

In this study polymerase chain reaction (PCR) was used to identify yeast in domestic ragi obtained from two local markets in Sarawak and Pahang. These ragi are normally used as a dry starter in food fermentation (tapai) for Pahang (ST2) and Sarawak (ST3) and tuak (ST1) which is an alcoholic drink in Sarawak. Universal primer, NL1 and NL4 were used as a primer in this study to amplify D1/D2 frag...

Phospholipase D (PLD) in plants plays vital roles in growth, development, and stress responses. However, the precise role of PLDs in pineapple remains poorly understood. In this study, 10 putative PLD genes, designated as AcPLD1-AcPLD10, were identified based on the pineapple genome database. The 10 AcPLDs could be clustered into five of the six known PLD families according to sequence characte...

The effects of intercycle cover crops on Rotylenchulus reniformis population densities in pineapple were evaluated in one greenhouse and two field experiments. In the greenhouse, Crotalaria juncea, Brassica napus, and Tagetes erecta were planted for 3 months and then incorporated. These treatments were compared to weedy fallow with or without 1,3-dichloropropene (1,3-D) in three soils (Makawao ...

The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis of 51 amplified bands. A dendrogram was created according to the 16 SSR marke...