Anopheles mosquitoes transmit at least 200 million annual malaria infections worldwide. Despite considerable genomic resources, mechanistic understanding of biological processes in Anopheles has been hampered by a lack of tools for reverse genetics. Here, we report successful application of the CRISPR/Cas9 system for highly efficient, site-specific mutagenesis in the diverse malaria vectors Ano...

Characterizing and monitoring biodiversity and assessing its drivers require accurate and comparable data on species assemblages, which, in turn, should rely on efficient and standardized field collection. Unfortunately, protocols that follow such criteria remain scarce and it is unclear whether they can be applied to megadiverse communities, whose study can be particularly challenging. Here, w...

Today’s highly competitive environment in the commercial air transportation business instigates airline companies to continue operating their aircrafts beyond the design life, as long as the maintenance ascertaining their safe operation is more cost-effective than the investment into a new aircraft. In the context of damage tolerance of aging aircrafts, the residual strength of the fuselage ski...

Automatic benchmarking can provide a reliable first insight into the accessibility status of a web site. The eGovMon project has developed a tool which can assess web sites according to a statistically sound sampling procedure. Additionally, the tool supports detailed evaluation of single web pages. This paper describes the process of data acquisition for the case of large scale accessibility b...

Medium-term modeling of electricity market has essential role in generation expansion planning. On the other hand, uncertainties strongly affect modeling and consequently, strategic analysis of generation firms in the medium term. Therefore, models considering these uncertainties are highly required. Among uncertain variables considered in the medium term generation planning, demand and hyd...

(1) The methodologies described in TMECC do not purport to address all safety concerns associated with their use. It is the responsibility of the user of these methods to establish appropriate safety and health practices, and to determine the applicability of regulatory limitations prior to their use. (2) All methods and sampling protocols provided in TMECC are subject to revision and update to...