Accurate spot detection and quantification is a challenging task that must be performed effectively in order to properly extract the proteomic information from two-dimensional (2-D) gel electrophoresis images. In Morris et al., Bioinformatics 24:529-536, 2008, we introduced Pinnacle, an automatic, fast, effective noncommercial package for spot detection and quantification for 2-D gel images, an...

For CISC microprocessors, the proportion of memory access instructions is relatively high, and a specific address is likely to be accessed repeatedly in a short period of time because of register-to-memory or memory-to-memory instruction set architectures and limited register sets. As superscalar architectures advance, an aggressive scheduling policy for memory access becomes crucial. In this p...

This paper describes the operation with the software environment called KSM (Knowledge Structure Manager) that supports a methodology for building and reusing knowledge models. The methodology is a useful tool for developers who need to build large and complex knowledge models in real world projects. The enviroment helps developers in applying the whole methodology to build the final system and...

This report examines properties of two main methods used for local background estimation in microarray images. The first method uses samples from local regions defined such that they supposedly contain only background pixels; we call this a region method. This method is implemented in the Axon GenePix Pro software. The second method uses filtering techniques that removes the bright spots from t...

BACKGROUND Manual counting of microvessels is subjective and may lead to unacceptable interobserver variability, which may explain conflicting results. AIMS To develop and test an automated method for microvessel counting and objective selection of the hot spot, based on image processing of whole sections, and to compare this with manual selection of a hot spot and counting of microvessels. ...

The present study examined the effects of boundary strength and stress on nasal coarticulation with neighboring segments. Acoustic and nasal airflow data were recorded from four speakers as they produced intervocalic fricative-nasal and nasalfricative sequences that spanned a word-internal boundary or a word boundary under two different stress conditions. Although neither stress nor boundary af...

Interactive method for optimization of an IC layout with respect to sensitivity to spot defects is presented. The relationship between occurrence of a spot defect and a circuit failure is discussed. A new concept of sensitive area is introduced. A new algorithm which allows determination of sensitive areas for opens is proposed. A practical example of the application of a software tool SENSAT t...

Heavy-duty trucks make up only 3% of the on-road vehicle fleet, yet they account for > 7% of vehicle miles traveled in the United States. They also contribute a significant proportion of regulated ambient emissions. Heavy vehicles emit emissions at different rates than passenger vehicles. They may also behave differently on-road, yet may be treated similarly to passenger vehicles in emissions m...